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Murine isolated lymphoid follicles contain follicular B lymphocytes with a mucosal phenotype
- Source :
- American Journal of Physiology-Gastrointestinal and Liver Physiology. 291:G595-G604
- Publication Year :
- 2006
- Publisher :
- American Physiological Society, 2006.
-
Abstract
- Isolated lymphoid follicles (ILFs) are organized intestinal lymphoid structures whose formation can be induced by luminal stimuli. ILFs have been demonstrated to act as inductive sites for the generation of immune responses directed toward luminal stimuli; however, the phenotype of the immune response initiated within ILFs has largely been uninvestigated. To gain a better understanding of the immune responses initiated within ILFs, we examined phenotypic and functional aspects of the largest cellular component of the murine ILF lymphocyte population, B lymphocytes. We observed that murine ILF B lymphocytes are composed of a relatively homogenous population of follicular B-2 B lymphocytes. Consistent with their proximity to multiple stimuli, ILF B lymphocytes displayed a more activated phenotype compared with their counterparts in the spleen and Peyer's patch (PP). ILF B lymphocytes also expressed higher levels of immunomodulatory B7 and CD28 family members B7X and programmed death-1 compared with their counterparts in the spleen and PP. ILF B lymphocytes preferentially differentiate into IgA-producing plasma cells and produce more IL-4 and IL-10 and less interferon-γ compared with their counterparts in the spleen. Immunoglobulin repertoire analysis from individual ILFs demonstrated that ILFs contain a polyclonal population of B lymphocytes. These findings indicate that murine ILFs contain a polyclonal population of follicular B-2 B lymphocytes with a phenotype similar to PP B lymphocytes and that, in unchallenged animals, ILFs promote immune responses with a homeostatic phenotype.
- Subjects :
- Immunoglobulin A
Lymphoid Tissue
Physiology
Cellular differentiation
Plasma Cells
Article
Mice
Antigens, CD
Physiology (medical)
Follicular phase
Animals
Immunity, Mucosal
Gene
Regulation of gene expression
B-Lymphocytes
Mice, Inbred BALB C
Hepatology
biology
Gastroenterology
Cell Differentiation
ANTIGENS CD
Phenotype
Cell biology
Lymphatic system
Gene Expression Regulation
Immunology
biology.protein
Cytokines
Female
Subjects
Details
- ISSN :
- 15221547 and 01931857
- Volume :
- 291
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Gastrointestinal and Liver Physiology
- Accession number :
- edsair.doi.dedup.....c5ef0dc93a327a7ff80aa8d2fa7dd67a
- Full Text :
- https://doi.org/10.1152/ajpgi.00525.2005