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Insights into the genetic diversity of an underutilized Indian legume, Vigna stipulacea (Lam.) Kuntz., using morphological traits and microsatellite markers
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0262634 (2022), PLoS ONE, Vol 17, Iss 1 (2022), PLoS ONE
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Vigna stipulacea (Lam.) Kuntz., commonly known as Minni payaru is an underutilized legume species and has a great potential to be utilized as food crop. To evaluate and select the best germplasm to be harnessed in the breeding programme, we assessed the genetic diversity of V. stipulacea (94 accessions) conserved in the Indian National Genebank, based on morphological traits and microsatellite markers. Significant variation was recorded for the morphological traits studied. Euclidean distance using UPGMA method grouped all accessions into two major clusters. Accessions were identified for key agronomic traits such as, early flowering (IC331436, IC251436, IC331437); long peduncle length (IC553518, IC550531, IC553557, IC553540, IC550532, IC553564); and more number of seeds per pod (IC553529, IC622865, IC622867, IC553528). To analyse the genetic diversity among the germplasm 33 SSR primers were used anda total of 116 alleles were detected. The number of alleles varied from two to seven, with an average of 3.52 per loci. The polymorphic information content values varied from 0.20 to 0.74, with a mean of 0.40. The high number of alleles per locus and the allelic diversity in the studied germplasm indicated a relatively wider genetic base of V. stipulacea. Phylogenetic analysis clustered accessions into seven clades. Population structure analysis grouped them into five genetic groups, which were partly supported by PCoA and phylogenetic tree. Besides, PCoA and AMOVA also decoded high genetic diversity among the V. stipulacea accessions. Thus, morphological and microsatellite markers distinguished V. stipulacea accessions and assessed their genetic diversity efficiently. The identified promising accessions can be utilized in Vigna improvement programme through introgression breeding and/or can be used for domestication and enhanced utilization of V. stipulacea.
- Subjects :
- Conservation Biology
Genotype
Science
India
Crops
Plant Science
Plant Genetics
Microsatellite Loci
Asian People
Gene Types
Genetics
Ethnicities
Phylogeny
Conservation Science
Crop Genetics
Evolutionary Biology
Multidisciplinary
Polymorphism, Genetic
Population Biology
Plant Anatomy
Vigna
Ecology and Environmental Sciences
Biology and Life Sciences
Genetic Variation
food and beverages
Agriculture
Fabaceae
Agronomy
Plant Breeding
Phenotype
People and Places
Seeds
Conservation Genetics
Medicine
Population Groupings
Tamil People
Population Genetics
Research Article
Crop Science
Microsatellite Repeats
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c5ef09bfc8e46a02a42d37ee30c0f0df