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Characterization of 4 T1-like lytic bacteriophages that lyse Shiga-toxin Escherichia coli O157:H7
- Source :
- Canadian Journal of Microbiology. 58:923-927
- Publication Year :
- 2012
- Publisher :
- Canadian Science Publishing, 2012.
-
Abstract
- Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC strains exhibiting extreme susceptibility (average multiplicity of infection (MOI) = 0.0003−0.0007). All phages had icosahedral heads with tapered, noncontractile tails, a morphology indicative of T1-like Siphoviridae. Genome size of all phages was ∼44 kb, but EcoRІ or HindIII digestion profiles differed among phages. Based on restriction enzyme digestion profiles, phages AHP24, AHS24, and AHP42 were more related (66.7%−82.4%) to each other than to AKS96, while AHP24 and AHS24, isolated from the same feedlot pen, exhibited the highest identity (88.9%−92.3%). Phages AHP24 and AHS24 exhibited the broadest host range and strongest lytic activity against STEC O157:H7, making them strong candidates for biocontrol of this bacterium in cattle.
- Subjects :
- viruses
Restriction Mapping
Immunology
Genome, Viral
HindIII
Escherichia coli O157
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
Host Specificity
Siphoviridae
Feces
fluids and secretions
Multiplicity of infection
Genetics
medicine
Animals
Bacteriophages
Molecular Biology
Escherichia coli
Genome size
Phylogeny
biology
Shiga toxin
DNA Restriction Enzymes
General Medicine
biology.organism_classification
Virology
Lytic cycle
biology.protein
Cattle
Bacteria
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....c5bd75521f907d10b4aeb07398e7de17