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Recombinant allergens promote expression of aminopeptidase-n (CD13) on basophils in allergic patients
- Source :
- International journal of immunopathology and pharmacology. 21(1)
- Publication Year :
- 2008
-
Abstract
- IgE-dependent activation of basophils is associated with upregulation of several surface molecules. We recently identified the surface enzyme aminopeptidase N (CD13) as a novel activation antigen on human basophils. In the present study, we asked whether CD13 can be employed as a novel marker of allergen-induced activation of basophils in allergic individuals. Patients allergic to major allergens from grass pollen (Phl p 1, Phl p 5), birch pollen (Bet v 1), or house dust mites (Der p 2), were examined. Blood basophils were exposed to various concentrations of recombinant allergens for 15 minutes, and examined for expression of CD13 by multicolor flow cytometry. The allergen-induced upregulation of CD13 was compared with allergen-dependent increases in expression of CD63 and CD203c. Exposure to recombinant allergens resulted in an increase in expression of CD13 on basophils in all sensitized individuals, whereas no increase in CD13 was seen in healthy controls. The effects of the recombinant allergens on CD13-expression were dose- and time-dependent, were not observed in the absence of extracellular calcium, and were counteracted by preincubation of basophils with the PI3-kinase-targeting drugs staurosporin and LY294002. There was a good correlation between allergen-induced upregulation of CD13, CD63, and CD203c on basophils. In aggregate, our data show that recombinant allergens promote expression of CD13 on basophils in sensitized individuals. The functional significance and diagnostic implications of this observation remain to be determined.
- Subjects :
- Adult
Male
Adolescent
Immunology
chemical and pharmacologic phenomena
Platelet Membrane Glycoproteins
CD13 Antigens
Flow cytometry
law.invention
chemistry.chemical_compound
Phosphatidylinositol 3-Kinases
Antigen
Downregulation and upregulation
law
Antigens, CD
parasitic diseases
otorhinolaryngologic diseases
medicine
Extracellular
Hypersensitivity
Immunology and Allergy
Humans
LY294002
Pyrophosphatases
Pharmacology
chemistry.chemical_classification
medicine.diagnostic_test
CD63
Chemistry
Phosphoric Diester Hydrolases
Tetraspanin 30
hemic and immune systems
Allergens
Middle Aged
Recombinant Proteins
respiratory tract diseases
Basophils
Enzyme
Recombinant DNA
Calcium
Female
Interleukin-3
Subjects
Details
- ISSN :
- 03946320
- Volume :
- 21
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of immunopathology and pharmacology
- Accession number :
- edsair.doi.dedup.....c5b33fcc475a82b2ca30c8dd162439cc