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Cellular response to high pulse repetition rate nanosecond pulses varies with fluorescent marker identity
- Source :
- Biochemical and Biophysical Research Communications. 478:1261-1267
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Nanosecond electric pulses (nsEP's) are a well-studied phenomena in biophysics that cause substantial alterations to cellular membrane dynamics, internal biochemistry, and cytoskeletal structure, and induce apoptotic and necrotic cell death. While several studies have attempted to measure the effects of multiple nanosecond pulses, the effect of pulse repetition rate (PRR) has received little attention, especially at frequencies greater than 100 Hz. In this study, uptake of Propidium Iodide, FM 1–43, and YO-PRO-1 fluorescent dyes in CHO-K1 cells was monitored across a wide range of PRRs (5 Hz–500 KHz) using a laser-scanning confocal microscope in order to better understand how high frequency repetition rates impact induced biophysical changes. We show that frequency trends depend on the identity of the dye under study, which could implicate transmembrane protein channels in the uptake response due to their chemical selectivity. Finally, YO-PRO-1 fluorescence was monitored in the presence of Gadolinium (Gd3+), Ruthenium Red, and in calcium-free solution to elucidate a mechanism for its unique frequency trend.
- Subjects :
- 0301 basic medicine
Ruthenium red
Time Factors
Confocal
Biophysics
Analytical chemistry
Gadolinium
Pyridinium Compounds
CHO Cells
Biochemistry
law.invention
03 medical and health sciences
chemistry.chemical_compound
Cricetulus
0302 clinical medicine
Confocal microscopy
law
Cricetinae
Fluorescence microscope
Animals
Humans
Propidium iodide
Molecular Biology
Fluorescent Dyes
Benzoxazoles
Pulse (signal processing)
Quinolinium Compounds
Cell Biology
Nanosecond
Ruthenium Red
Fluorescence
Quaternary Ammonium Compounds
Spectrometry, Fluorescence
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Nanoparticles
Calcium
Propidium
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 478
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....c59ebe0b479849b87388495a18fa5a0d