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An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70
- Source :
- University of Copenhagen
- Publication Year :
- 1985
- Publisher :
- Elsevier BV, 1985.
-
Abstract
- This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
- Subjects :
- Paper
Immunodiffusion
animal structures
Immunology
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Autoantigens
behavioral disciplines and activities
Absorption
Antigen
immune system diseases
hemic and lymphatic diseases
Collodion
medicine
Animals
Lupus Erythematosus, Systemic
Immunology and Allergy
Antigens
Nuclear protein
Autoantibodies
chemistry.chemical_classification
Scleroderma, Systemic
biology
Chemistry
fungi
Autoantibody
Antigens, Nuclear
Molecular biology
Rats
Cell nucleus
Nucleoproteins
medicine.anatomical_structure
Enzyme
biology.protein
Electrophoresis, Polyacrylamide Gel
Antibody
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Journal of Immunological Methods
- Accession number :
- edsair.doi.dedup.....c59ae27e17a97720e17f1d3194a6588f
- Full Text :
- https://doi.org/10.1016/0022-1759(85)90022-5