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Dihydroartemisinin represses oral squamous cell carcinoma progression through downregulating mitochondrial calcium uniporter
- Source :
- Bioengineered, Vol 13, Iss 1, Pp 227-241 (2022), Bioengineered, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Dysregulation of mitochondrial calcium uniporter (MCU) exerts a carcinogenic effect in several cancers. Nevertheless, the roles of MCU in oral squamous cell carcinoma (OSCC) remain elusive. It has been reported that Dihydroartemisinin (DHA) may suppress the progression of OSCC but its associated mechanisms have been not investigated. The purpose of our research was to observe the biological function of MCU on OSCC and its regulatory relationship with DHA. MCU, MICU1, MICU2, N-cadherin, TGF-β and Vimentin expression was detected in OSCC and peritumoral tissues by immunohistochemistry and western blot. Following DHA treatment, the expression of above proteins was detected in CAL-27 cells transfected with shMCU or pcDNA3.1-MCU by western blot or immunofluorescence. Furthermore, clone formation, mitochondrial membrane potential (MMP), wound healing and transwell assays were presented in CAL-27 cells treated with DHA, shMCU or pcDNA3.1-MCU. Our results showed that the members of MCU complex including MCU, MICU1 and MICU2 were overexpressed in OSCC compared to peritumoral tissues. Furthermore, TGF-β and epithelial to mesenchymal transition (EMT) proteins (N-cadherin and Vimentin) exhibited higher expression in OSCC. DHA treatment significantly lowered the expression of MCU in CAL-27 cells. MCU overexpression reversed the inhibitory effects of DHA on MICU1, MICU2, N-cadherin, TGF-β and Vimentin expression. Furthermore, MCU knockdown or DHA suppressed proliferation, MMP and migration of CAL-27 cells. DHA treatment could reverse the effects of MCU overexpression. Collectively, our study demonstrated that MCU was an oncogene of OSCC and DHA exerted a suppressive role on proliferation and migration of OSCC cells by suppressing MCU expression.
- Subjects :
- Male
Epithelial-Mesenchymal Transition
proliferation
Clone (cell biology)
Down-Regulation
Bioengineering
Vimentin
migration
Mitochondrial Membrane Transport Proteins
Applied Microbiology and Biotechnology
dihydroartemisinin
Western blot
Cell Movement
Cell Line, Tumor
Biomarkers, Tumor
medicine
Humans
Epithelial–mesenchymal transition
Cation Transport Proteins
Cell Proliferation
Membrane Potential, Mitochondrial
Gene knockdown
Oncogene
biology
medicine.diagnostic_test
Squamous Cell Carcinoma of Head and Neck
Chemistry
Calcium-Binding Proteins
General Medicine
Transfection
Artemisinins
Gene Expression Regulation, Neoplastic
oral squamous cell carcinoma
stomatognathic diseases
mitochondrial calcium uniporter
Disease Progression
biology.protein
Cancer research
Immunohistochemistry
Female
Mouth Neoplasms
lipids (amino acids, peptides, and proteins)
Calcium Channels
TP248.13-248.65
Research Article
Research Paper
Biotechnology
Subjects
Details
- ISSN :
- 21655987 and 21655979
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Bioengineered
- Accession number :
- edsair.doi.dedup.....c58f7033cb7a2f2567d14d9014d521cc