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Genome-wide identification of CCT genes in wheat (Triticum aestivum L.) and their expression analysis during vernalization
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0262147 (2022), PLoS ONE, Vol 17, Iss 1 (2022), PLoS ONE
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Numerous CCT genes are known to regulate various biological processes, such as circadian rhythm regulation, flowering, light signaling, plant development, and stress resistance. The CCT gene family has been characterized in many plants but remains unknown in the major cereal wheat (Triticum aestivum L.). Extended exposure to low temperature (vernalization) is necessary for winter wheat to flower successfully. VERNALIZATION2 (VRN2), a specific CCT-containing gene, has been proved to be strongly associated with vernalization in winter wheat. Mutation of all VRN2 copies in three subgenomes results in the eliminated demands of low temperature in flowering. However, no other CCT genes have been reported to be associated with vernalization to date. The present study screened CCT genes in the whole wheat genome, and preliminarily identified the vernalization related CCT genes through expression analysis. 127 CCT genes were identified in three subgenomes of common wheat through a hidden Markov model-based method. Based on multiple alignment, these genes were grouped into 40 gene clusters, including the duplicated gene clusters TaCMF6 and TaCMF8, each tandemly arranged near the telomere. The phylogenetic analysis classified these genes into eight groups. The transcriptome analysis using leaf tissues collected before, during, and after vernalization revealed 49 upregulated and 31 downregulated CCT genes during vernalization, further validated by quantitative real-time PCR. Among the differentially expressed and well-investigated CCT gene clusters analyzed in this study, TaCMF11, TaCO18, TaPRR95, TaCMF6, and TaCO16 were induced during vernalization but decreased immediately after vernalization, while TaCO1, TaCO15, TaCO2, TaCMF8, and TaPPD1 were stably suppressed during and after vernalization. These data imply that some vernalization related CCT genes other than VRN2 may exist in wheat. This study improves our understanding of CCT genes and provides a foundation for further research on CCT genes related to vernalization in wheat.
- Subjects :
- genetic structures
Physiology
Gene Expression
Plant Science
Biochemistry
Gene Expression Regulation, Plant
Phylogeny
Triticum
Plant Proteins
Data Management
Multidisciplinary
Chromosome Mapping
Eukaryota
food and beverages
Phylogenetic Analysis
Genomics
Plants
Cold Temperature
Phylogenetics
Experimental Organism Systems
Multigene Family
Plant Physiology
Wheat
Medicine
Transcriptome Analysis
Research Article
Computer and Information Sciences
Photoperiod
Arabidopsis Thaliana
Science
Flowers
Brassica
Research and Analysis Methods
Model Organisms
Vernalization
Protein Domains
Plant and Algal Models
Genetics
Evolutionary Systematics
Grasses
Taxonomy
Evolutionary Biology
Whole Genome Sequencing
Sequence Analysis, RNA
Gene Expression Profiling
Organisms
Biology and Life Sciences
Computational Biology
Proteins
Genome Analysis
eye diseases
Plant Leaves
Animal Studies
Rice
sense organs
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c587b6fac7ae2707234fca96f9bad1d7