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Extracellular-Vesicle Isolation from Different Biological Fluids by Size-Exclusion Chromatography
- Source :
- Current protocols in stem cell biology. 49(1)
- Publication Year :
- 2019
-
Abstract
- This unit describes how to isolate extracellular vesicles (EVs) from different biological fluids using size-exclusion chromatography (SEC) and how to prepare your starting sample and the EV product for downstream applications. EVs are membrane nanovesicles with specific content that reflects the phenotype and functions of the cell of origin, including protected proteins, lipids, metabolites, and nucleic acids. EVs are thus an excellent resource for noninvasive biomarker discovery in a number of pathological situations and are a promising nanotherapeutic tool to overcome the disadvantages associated with cellular therapy. However, there are still no standardized methods to isolate pure EV preparations, as many approaches do not guarantee proper EV purification, free of contaminating non-EV molecules. Currently, SEC is one of the most promising approaches to purify EVs from any biological fluid, as it avoids co-isolation of contaminants and is user friendly and scalable. © 2019 by John Wiley & Sons, Inc.
- Subjects :
- 0301 basic medicine
Size-exclusion chromatography
Cell Biology
General Medicine
Extracellular vesicle
Biology
Exosome
Biological fluid
Body Fluids
03 medical and health sciences
Extracellular Vesicles
030104 developmental biology
0302 clinical medicine
Membrane
Biochemistry
Nucleic acid
Biological fluids
Chromatography, Gel
Humans
030217 neurology & neurosurgery
Cells, Cultured
Developmental Biology
Noninvasive biomarkers
Subjects
Details
- ISSN :
- 19388969
- Volume :
- 49
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Current protocols in stem cell biology
- Accession number :
- edsair.doi.dedup.....c56cdef585545b197f714812fc4d21b9