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Administration of osteocalcin accelerates orthodontic tooth movement induced by a closed coil spring in rats
- Source :
- European journal of orthodontics. 23(5)
- Publication Year :
- 2001
-
Abstract
- The effect of local administration of osteocalcin (OC) on experimental tooth movement was examined in the rat. The maxillary first molar was first moved mesially with an initial tipping force of 30 g with a closed-coil spring anchored to the incisor for 10 days (n = 48). Three experimental groups (n = 8) were injected with purified rat OC at doses of 0.1, 1, and 10 micrograms, respectively. The injection into the palatal bifurcation site of the first molar was repeated daily. The control groups (n = 8) were injected with rat serum albumin (10 micrograms), phosphate buffered saline (PBS), or were not injected. Tooth movement was evaluated daily by measuring the inter-cuspal distance between the first and the second molars on a precise plaster model. The cumulative tooth movement (mm) in the 1-microgram OC-injected groups was significantly more than that in all of the control groups on day 9. The rate of tooth movement (mm/day) showed periodical elevation, with high values on days 1, 4, 7, and 9. Acceleration of tooth movement by OC was significant in the early experimental period. Subsequently, acceleration of early tooth movement by OC was histologically evaluated (n = 40). Each of four animals from the control (PBS, n = 20) and the experimental (1 microgram OC, n = 20) groups was killed daily up to 5 days. A significantly larger number of osteoclasts accumulated on the mesial alveolar bone surface in the 1-microgram OC-injected group on day 3 than that observed in control group. These results suggest that administration of OC accelerates orthodontic tooth movement due to enhancement of osteoclastogenesis on the pressure side, primarily in the early experimental period.
- Subjects :
- Molar
Male
Microinjections
Tooth Movement Techniques
Acid Phosphatase
Osteocalcin
Statistics as Topic
Serum albumin
Dentistry
Osteoclasts
Orthodontics
Cell Count
Sodium Chloride
Mandibular first molar
Mandibular second molar
stomatognathic system
Incisor
medicine
Maxillary first molar
Alveolar Process
Orthodontic Wires
Animals
Tolonium Chloride
Rats, Wistar
Coloring Agents
Dental alveolus
Serum Albumin
Analysis of Variance
Osteoblasts
biology
business.industry
Chemistry
Palate
Tartrate-Resistant Acid Phosphatase
Models, Dental
Rats
Isoenzymes
stomatognathic diseases
medicine.anatomical_structure
biology.protein
Bone Remodeling
business
Biomarkers
Follow-Up Studies
Subjects
Details
- ISSN :
- 01415387
- Volume :
- 23
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- European journal of orthodontics
- Accession number :
- edsair.doi.dedup.....c555179b0871fce7fe61c4f1c26ea335