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Protective capacities of cell surface-associated proteins of Streptococcus suis mutants deficient in divalent cation-uptake regulators
- Source :
- Microbiology. 155:1580-1587
- Publication Year :
- 2009
- Publisher :
- Microbiology Society, 2009.
-
Abstract
- Many cell surface-associated, divalent cation-regulated proteins are immunogenic, and some of them confer protection against the bacterial species from which they are derived. In this work, two Streptococcus suis divalent cation uptake regulator genes controlling zinc/manganese and iron uptake (adcR and fur, respectively) were inactivated in order to study the protective capacities of their cell surface-associated proteins. The results obtained showed overexpression of a set of immunogenic proteins (including members of the pneumococcal histidine triad family previously reported to confer protection against streptococcal pathogens) in S. suis adcR mutant cell surface extracts. Likewise, genes encoding zinc transporters, putative virulence factors and a ribosomal protein paralogue related to zinc starvation appeared to be derepressed in this mutant strain. Moreover, protection assays in mice showed that although neither adcR- nor fur-regulated cell surface-associated proteins were sufficient to confer protection in mice, the combination of both adcR- and fur-regulated cell surface-associated proteins is able to confer significant protection (50 %, P=0.038) against a challenge to mice vaccinated with them.
- Subjects :
- Streptococcus suis
Cations, Divalent
Cell
Mutant
Virulence
Biology
Microbiology
Divalent
Mice
Bacterial Proteins
Ribosomal protein
Streptococcal Infections
medicine
Animals
Humans
Gene
Histidine
chemistry.chemical_classification
Mice, Inbred BALB C
Membrane Proteins
Biological Transport
Gene Expression Regulation, Bacterial
biology.organism_classification
Repressor Proteins
medicine.anatomical_structure
Biochemistry
chemistry
Mutation
Female
Transcription Factors
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 155
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....c54cddcd63e5142ce3eb5da47b066446