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Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
- Source :
- Stem Cell Research, Vol 20, Iss C, Pp 105-114 (2017), De Sousa, P A, Steeg, R, Wachter, E, Bruce, K, King, J, Hoeve, M, Khadun, S, Mcconnachie, G, Holder, J, Kurtz, A, Seltmann, S, Dewender, J, Reimann, S, Stacey, G, O'shea, O, Chapman, C, Healy, L, Zimmermann, H, Bolton, B, Rawat, T, Atkin, I, Veiga, A, Kuebler, B, Serano, B M, Saric, T, Hescheler, J, Brüstle, O, Peitz, M, Thiele, C, Geijsen, N, Holst, B, Clausen, C, Lako, M, Armstrong, L, Gupta, S K, Kvist, A J, Hicks, R, Jonebring, A, Brolén, G, Ebneth, A, Cabrera-socorro, A, Foerch, P, Geraerts, M, Stummann, T C, Harmon, S, George, C, Streeter, I, Clarke, L, Parkinson, H, Harrison, P W, Faulconbridge, A, Cherubin, L, Burdett, T, Trigueros, C, Patel, M J, Lucas, C, Hardy, B, Predan, R, Dokler, J, Brajnik, M, Keminer, O, Pless, O, Gribbon, P, Claussen, C, Ringwald, A, Kreisel, B, Courtney, A & Allsopp, T E 2017, ' Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) : The Hot Start experience ', Stem Cell Research, vol. 20, pp. 105-114 . https://doi.org/10.1016/j.scr.2017.03.002, Stem Cell Research, 20, 105. Elsevier, Stem Cell Research, 20, 105, Stem Cell Research, 20, 105-114, NARCIS, BASE (Open Access Aggregator), UnpayWall, DOAJ-Articles, ORCID, Microsoft Academic Graph, Stem Cell Research, 20, 105-114. Elsevier B.V., Stem Cell Research
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- A fast track “Hot Start” process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community.eTOCThe report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.
- Subjects :
- 0301 basic medicine
QH301-705.5
Process (engineering)
media_common.quotation_subject
Induced Pluripotent Stem Cells
Biology
Tissue procurement
Cell Line
03 medical and health sciences
Resource (project management)
Journal Article
Humans
Quality (business)
Biology (General)
Induced pluripotent stem cell
lcsh:QH301-705.5
Biological Specimen Banks
media_common
Cryopreservation
International research
Medicine(all)
Hot start
General Medicine
Cell Biology
Europe
Engineering management
030104 developmental biology
lcsh:Biology (General)
Work flow
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18767753 and 18735061
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....c54bb6b7d2c9e8f837df5a8697d4288a