Back to Search
Start Over
The effect of dietary estimates calculated using food frequency questionnaires on micronuclei formation in European pregnant women: a NewGeneris study
- Source :
- Mutagenesis, 29(6), 393-400. Oxford University Press
- Publication Year :
- 2014
-
Abstract
- The use of biomarkers of early genetic effects, predictive for cancer, such as micronuclei (MN) in lymphocytes, may help to investigate the association between diet and cancer. We hypothesised that the presence of mutagens in the diet may increase MN formation. A 'pooled' standardised analysis was performed by applying the same experimental protocol for the cytokinesis block micronucleus assay in 625 young healthy women after delivery from five European study populations (Greece, Denmark, UK, Spain and Norway). We assessed MN frequencies in mono- and binucleated T-lymphocytes (MNMONO and MNBN) and the cytokinesis blocked proliferation index using a semi-automated image analysis system. Food frequency questionnaires (FFQs) were used to estimate intake of fatty acids and a broad range of immunotoxic and genotoxic/carcinogenic compounds through the diet. Pooled difference based on delivery type revealed higher MNMONO frequencies in caesarean than in vaginal delivery (P = 0.002). Statistical analysis showed a decrease in MNMONO frequencies with increasing calculated omega-6 PUFA concentrations and a decrease in MNBN frequencies with increasing calculated omega-3 PUFA concentrations. The expected toxic compounds estimated by FFQs were not associated with MN formation in mothers after delivery. In pregnant women, an omega-3 and -6 rich diet estimated by FFQ is associated with lower MN formation during pregnancy and delivery.
- Subjects :
- Adult
Mitotic index
Proliferation index
T-Lymphocytes
Health, Toxicology and Mutagenesis
Physiology
Toxicology
White People
Cohort Studies
Diet and cancer
Surveys and Questionnaires
Genetics
medicine
Humans
Micronuclei, Chromosome-Defective
Genetics (clinical)
Cell Proliferation
Cytokinesis
chemistry.chemical_classification
Pregnancy
Vaginal delivery
business.industry
Feeding Behavior
Middle Aged
medicine.disease
Diet
chemistry
Micronucleus test
Immunology
Linear Models
Female
pregnancy
business
Mutagens
Polyunsaturated fatty acid
Cohort study
Subjects
Details
- Language :
- English
- ISSN :
- 02678357
- Database :
- OpenAIRE
- Journal :
- Mutagenesis, 29(6), 393-400. Oxford University Press
- Accession number :
- edsair.doi.dedup.....c51fa5bfd531999c24c011fd52c63311