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Potential prophylactic effect of recombinant bovine somatotropin (rbST) in sheep with experimentally induced hyperketonemia
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Hyperketonemia in sheep is associated with a disease known as pregnancy toxemia. The purposes of this study were to (1) induce hyperketonemia by infusion with D-beta-hydroxybutyrate (D-BHB) in eighteen healthy, non-pregnant, non-lactating ewes, (2) to evaluate the metabolic pathways in which recombinant bovine somatotropin (rbST) may be involved, and (3) to evaluate the possible benefits of preventive rbST therapy on the metabolic profiles of the sheep. The sheep were intravenously infused with a D-BHB solution over 2 h. Three days prior to the infusion, one group (n = 9) was injected with a single dose of 160 mg rbST, and the other group (n = 9) was injected with saline as a control. Blood samples were collected and metabolic profiles were analyzed every 20 min during the infusion and at 15, 30, 60, 120, 180, and 240 min post-infusion. Hyperketonemia was successfully achieved, demonstrated by serum BHB concentrations of 3.9 and 3.0 mmol/L in the rbST and control groups, respectively, without the presence of clinical signs. An increase in peripheral insulin resistance was observed after infusion but not during the infusion, in the rbST group. Higher glucose concentrations were observed in the rbST group post-infusion, suggesting a positive impact of D-BHB on gluconeogenesis. Our data suggest that prophylactic treatment with rbST may be useful in sheep to reduce the risk of PT.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
040301 veterinary sciences
medicine.medical_treatment
Sheep Diseases
law.invention
0403 veterinary science
03 medical and health sciences
law
Internal medicine
Animals
Insulin
Medicine
Bovine somatotropin
Insulin-Like Growth Factor I
Saline
Pregnancy
Sheep
3-Hydroxybutyric Acid
General Veterinary
business.industry
Ketosis
04 agricultural and veterinary sciences
Peripheral insulin resistance
medicine.disease
Recombinant Proteins
030104 developmental biology
Endocrinology
Gluconeogenesis
Growth Hormone
Recombinant DNA
Cattle
Female
business
OVINOS
Metabolic profile
Prophylactic treatment
Subjects
Details
- ISSN :
- 00345288
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Research in Veterinary Science
- Accession number :
- edsair.doi.dedup.....c519d46dff5367fada2d7dcd30ca94ff