Back to Search
Start Over
In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression
- Source :
- Journal of Experimental & Clinical Cancer Research : CR, Journal of Experimental & Clinical Cancer Research, Vol 29, Iss 1, p 13 (2010)
- Publication Year :
- 2010
- Publisher :
- BioMed Central, 2010.
-
Abstract
- Background Genome-wide RNA interference screening study revealed that loss of expression of insulin-like growth factor binding protein 7 (IGFBP7) is a critical step in development of a malignant melanoma (MM), and this secreted protein plays a central role in apoptosis of MM. In this study we constructed pcDNA3.1-IGFBP7 to obtain high expression of IGBPF7 and to inhibit the growth of MM in C57BL/6J mice. Methods pcDNA3.1-IGFBP7 was transfected into B16-F10 cell, the expression of IGFBP7 was detected by RT-PCR and western blot. The proliferations and apoptosis rates of transfected and control cells were measured by CCK8 and FCM, respectively. The tumorigenicity and tumor growth in both pcDNA3.1-IGFBP7 group and control groups were studied in C57BL/6J mice model. IGFBP7, caspase-3, and VEGF expressions in tumor tissue were measured by immunohistochemistry. Apoptosis of tumors were detected by TUNEL. Results We demonstrated this plasmid inhibited proliferation of B16-F10 melanoma cells efficiently in vivo, exploiting the high expression of IGFBP7. More importantly, in-vivo transfection of pcDNA3.1-IGFBP7 inhibited MM growth in C57BL/6J mice. The inhibition of MM growth was proved owing to apoptosis and reduced expression of VEGF induced by pcDNA3.1-IGFBP7. Conclusions These results suggest a potential new clinical strategy for MM gene treatment.
- Subjects :
- Cancer Research
DNA, Complementary
Skin Neoplasms
IGFBP7
Cell Survival
medicine.medical_treatment
Cell
Melanoma, Experimental
Apoptosis
Transfection
lcsh:RC254-282
Insulin-like growth factor-binding protein
Mice
Western blot
In vivo
medicine
Animals
biology
medicine.diagnostic_test
Vascular Endothelial Growth Factors
Growth factor
Research
Genetic Therapy
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Molecular biology
Insulin-Like Growth Factor Binding Proteins
medicine.anatomical_structure
Oncology
biology.protein
Cancer research
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 17569966 and 03929078
- Volume :
- 29
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research : CR
- Accession number :
- edsair.doi.dedup.....c5162ec42e4430fd7d473c3183f4aeff