Back to Search
Start Over
In VivoMagnetic Resonance Imaging and Semiautomated Image Analysis Extend the Brain Phenotype forcdf/cdfMice
- Source :
- The Journal of Neuroscience. 26:4455-4459
- Publication Year :
- 2006
- Publisher :
- Society for Neuroscience, 2006.
-
Abstract
- Magnetic resonance imaging and computer image analysis in human clinical studies effectively identify abnormal neuroanatomy in disease populations. As more mouse models of neurological disorders are discovered, such an approach may prove useful for translational studies. Here, we demonstrate the effectiveness of a similar strategy for mouse neuroscience studies by phenotyping mice with the cerebellar deficient folia (cdf) mutation. Usingin vivomultiple-mouse magnetic resonance imaging for increased throughput, we imaged groups ofcdfmutant, heterozygous, and wild-type mice and made an atlas-based segmentation of the structures in 15 individual brains. We then performed computer automated volume measurements on the structures. We found a reduced cerebellar volume in thecdfmutants, which was expected, but we also found a new phenotype in the inferior colliculus and the olfactory bulbs. Subsequent local histology revealed additional cytoarchitectural abnormalities in the olfactory bulbs. This demonstrates the utility of anatomical magnetic resonance imaging and semiautomated image analysis for detecting abnormal neuroarchitecture in mutant mice.
- Subjects :
- Male
Inferior colliculus
Cerebellum
Pathology
medicine.medical_specialty
Mutant
Contrast Media
Mice, Transgenic
Biology
Mice
Mice, Neurologic Mutants
Imaging, Three-Dimensional
Chlorides
In vivo
Image Interpretation, Computer-Assisted
medicine
Animals
medicine.diagnostic_test
General Neuroscience
Brain
Magnetic resonance imaging
Articles
Magnetic Resonance Imaging
Phenotype
medicine.anatomical_structure
Manganese Compounds
Cytoarchitecture
Female
Neuroscience
Neuroanatomy
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....c5026603b32dac48d26e05daa10da03b