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Trans‐Oral Brush Biopsies and Quantitative PCR for EBV DNA Detection and Screening of Nasopharyngeal Carcinoma
- Source :
- Otolaryngology–Head and Neck Surgery. 150:602-609
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- To evaluate a newly developed noninvasive ambulatory, quantitative polymerase chain reaction (Q-PCR) Epstein-Barr virus (EBV) DNA detection and screening system (NP Screen™) for nasopharyngeal carcinoma (NPC).Correlation of the nasopharyngeal epithelial EBV-DNA levels and clinical findings by nasopharyngoscopy and final pathologic diagnosis of NPC with biopsy.Multicenter ENT/Oncology clinics in Hong Kong (Radiation Oncology Clinic at the Queen Elizabeth Hospital and Radiation Oncology Clinic and Head and Neck Clinic, Queen Mary Hospital, University of Hong Kong) and in Toronto, Canada (the Otolaryngology-Head and Neck Clinic at the Rouge Valley Health System and 2 large ENT practices in Toronto).A single-use trans-oral brush was used for rapid, nontraumatic nasopharyngeal (NP) epithelial cells DNA harvest in 600 Chinese patients, combined with a preservation and shipping kit for remote, real-time Q-PCR EBV DNA determinations.All 600 patients had NP brushings using NP Screen in an ambulatory environment, and no adverse events or complications were recorded. A final 578 patients were included with sufficient amount of DNA for completion of the Q-PCR assay. Of these 578 patients, 94 were confirmed positive for NPC histologically. The study yielded a sensitivity of 98.9%, specificity of 99.3%, positive predictive value (PPV) of 96.9%, and negative predictive value (NPP) of 99.7% for NP Screen in detecting NPC. Endoscopy had a sensitivity of 94%, specificity 97.1%, PPV 85%, and NPP 98.9%.The trans-oral brushing system fulfills the characteristics of a noninvasive, sensitive, specific detection method suitable for routine, large-scale ambulatory NPC risk assessment for high-risk NPC populations.
- Subjects :
- Male
Oncology
Epstein-Barr Virus Infections
Herpesvirus 4, Human
medicine.medical_specialty
Pathology
Biopsy
Nasopharyngoscopy
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Sensitivity and Specificity
Statistics, Nonparametric
Virus
Cohort Studies
Internal medicine
Radiation oncology
Ambulatory Care
medicine
Humans
Head and neck
Early Detection of Cancer
Retrospective Studies
Nasopharyngeal Carcinoma
Laryngoscopy
business.industry
Carcinoma
Mouth Mucosa
Nasopharyngeal Neoplasms
Viral Load
medicine.disease
Epstein–Barr virus
Dna detection
Microscopy, Electron
Real-time polymerase chain reaction
Otorhinolaryngology
Nasopharyngeal carcinoma
DNA, Viral
Female
Surgery
business
Subjects
Details
- ISSN :
- 10976817 and 01945998
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Otolaryngology–Head and Neck Surgery
- Accession number :
- edsair.doi.dedup.....c4ed4ded59b406e483c9fb72c38d8236