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Detection of G-quadruplex DNA in mammalian cells
- Source :
- Nucleic Acids Research, Nucleic Acids Research, 42(2), 860-869. Oxford University Press
- Publication Year :
- 2017
-
Abstract
- It has been proposed that guanine-rich DNA forms four-stranded structures in vivo called G-quadruplexes or G4 DNA. G4 DNA has been implicated in several biological processes, but tools to study G4 DNA structures in cells are limited. Here we report the development of novel murine monoclonal antibodies specific for different G4 DNA structures. We show that one of these antibodies designated 1H6 exhibits strong nuclear staining in most human and murine cells. Staining intensity increased on treatment of cells with agents that stabilize G4 DNA and, strikingly, cells deficient in FANCJ, a G4 DNA-specific helicase, showed stronger nuclear staining than controls. Our data strongly support the existence of G4 DNA structures in mammalian cells and indicate that the abundance of such structures is increased in the absence of FANCJ. We conclude that monoclonal antibody 1H6 is a valuable tool for further studies on the role of G4 DNA in cell and molecular biology.
- Subjects :
- 0301 basic medicine
Expression of Concern
medicine.drug_class
DNA repair
Biology
Gene Regulation, Chromatin and Epigenetics
010402 general chemistry
Monoclonal antibody
G-quadruplex
SEQUENCE
01 natural sciences
Chromosomes
SACCHAROMYCES-CEREVISIAE
03 medical and health sciences
chemistry.chemical_compound
Mice
Genetics
medicine
Animals
Humans
CRYSTAL-STRUCTURE
NUCLEIC-ACIDS
OXYTRICHA TELOMERIC DNA
030304 developmental biology
Cell Nucleus
0303 health sciences
BINDING PROTEINS
Helicase
Antibodies, Monoclonal
DNA
Molecular biology
Fanconi Anemia Complementation Group Proteins
Cell biology
0104 chemical sciences
3. Good health
Proliferating cell nuclear antigen
G-Quadruplexes
Cell nucleus
030104 developmental biology
medicine.anatomical_structure
ANTIBODY
chemistry
Nucleic acid
biology.protein
COMPLEXES
GENOMIC STABILITY
G-QUARTET
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 45
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....c4ea048f170f048fbe0f26d54c4148b8