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Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components
- Source :
- Cytotherapy, Cytotherapy; Vol 14
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Background aims The clinical use of human mesenchymal stromal cells (MSC) requires ex vivo expansion in media containing supplements such as fetal bovine serum or, alternatively, human platelet lysate (PL). Methods Platelet concentrates were frozen, quarantine stored, thawed and sterile filtered to obtain PL. PL content and its effect on fibroblast-colony-forming unit (CFU-F) formation, MSC proliferation and large-scale expansion were studied. Results PL contained high levels of basic fibroblast growth factor (bFGF), soluble CD40L (sCD40L), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), platelet-derived growth factor AA (PDGF-AA), platelet-derived growth factor AB/BB (PDGF-AB/BB), chemokine (C-C) ligand 5 (CCL5; RANTES) transforming growth factor-β1 (TGF-β1) and chemokine (C-X-C) ligand 1/2/3 (GRO), with low batch-to-batch variability, and most were stable for up to 14 days. Inhibition of PDGF-BB and bFGF decreased MSC proliferation by about 20% and 50%, respectively. The strongest inhibition (about 75%) was observed with a combination of anti-bFGF + anti-PDGF-BB and anti-bFGF + anti-TGF-β1 + anti-PDGF-BB. Interestingly, various combinations of recombinant PDGF-BB, bFGF and TGF-β1 were not sufficient to promote cell proliferation. PL from whole blood-derived pooled platelet concentrates and apheresis platelet concentrates did not differ significantly in their growth-promoting activity on MSC. Conclusions PL enhances MSC proliferation and can be regarded as a safe tool for MSC expansion for clinical purposes. \in particular, PDGF-BB and bFGF are essential components for the growth-promoting effect of PL, but are not sufficient for MSC proliferation.
- Subjects :
- Blood Platelets
Cancer Research
Stromal cell
Immunology
Becaplermin
chemokines
Bone Marrow Cells
Biology
03 medical and health sciences
0302 clinical medicine
Animals
Humans
Immunology and Allergy
Platelet
Genetics (clinical)
mesenchymal progenitor cells
Cell Proliferation
030304 developmental biology
Whole blood
0303 health sciences
Transplantation
Mesenchymal stem cell
Mesenchymal Stem Cells
Proto-Oncogene Proteins c-sis
Cell Biology
Fibroblasts
cytokines
Recombinant Proteins
3. Good health
Apheresis
Oncology
030220 oncology & carcinogenesis
Blood Component Removal
Cancer research
Original Article
Fibroblast Growth Factor 2
Platelet lysate
Stem cell
mesenchymal stromal cells
Fetal bovine serum
Subjects
Details
- ISSN :
- 14653249
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Cytotherapy
- Accession number :
- edsair.doi.dedup.....c4d4d07550db6dc5e36f7648e5a9b9ec