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Serotyping of naturally Toxoplasma gondii infected meat-producing animals
- Source :
- Veterinary Parasitology, Veterinary Parasitology, Elsevier, 2010, 169 (1-2), pp.24-8. ⟨10.1016/j.vetpar.2009.12.025⟩
- Publication Year :
- 2009
-
Abstract
- International audience; Serotyping was previously described as a promising method for typing strains of Toxoplasma gondii. The majority of precedent studies utilized serum samples collected from human patients with different T. gondii-associated pathologies. The aim of this work was to study the applicability of the same procedure for serotyping naturally infected meat-producing animals. An ELISA test based on GRA6 and GRA7 C-terminal polymorphic peptides was used. Peptide GRA6II has polymorphisms specific for the archetypal strains type II, GRA6I/III for strains type I and III, GRA7I for strains type I and GRA7III for strains type III. As reference material, and to validate this approach, serum samples from eleven free-range chickens and fifteen pigs used for Toxoplasma genotypes isolation were selected. These strains integrate the Biological Resource Centre (BRC) ToxoBS Bank. Three serum samples from chickens and two from pigs had serotyping results in agreement with genotyping. Thirty-five serum samples from chickens, twenty-nine from pigs and fifty from sheep, seropositive for T. gondii, from which no isolate was obtained, were also serotyped. Serotype III appeared significantly more frequent among sheep. Our results show that serotyping still need refinement, but may become a valuable tool for typing Toxoplasma strains from animal origin.
- Subjects :
- Serotype
MESH: Food Parasitology
Protozoan Proteins
Antibodies, Protozoan
Animal origin
MESH: Genotype
0302 clinical medicine
Food Parasitology
Genotype
MESH: Animals
MESH: Protozoan Proteins
MESH: Meat
0303 health sciences
MESH: Toxoplasma
MESH: Enzyme-Linked Immunosorbent Assay
General Medicine
MESH: Reproducibility of Results
MESH: Portugal
MESH: Toxoplasmosis
Toxoplasma
Toxoplasmosis
Meat
030231 tropical medicine
Toxoplasma gondii
Antigens, Protozoan
Enzyme-Linked Immunosorbent Assay
Biology
Microbiology
03 medical and health sciences
parasitic diseases
MESH: Antibodies, Protozoan
Animals
Typing
Serotyping
Genotyping
Infecções Sistémicas e Zoonoses
General Veterinary
Portugal
030306 microbiology
MESH: Serotyping
Reproducibility of Results
Serum samples
biology.organism_classification
Virology
Parasitology
Meat-producing-animals
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
MESH: Antigens, Protozoan
Subjects
Details
- ISSN :
- 18732550 and 03044017
- Volume :
- 169
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Veterinary parasitology
- Accession number :
- edsair.doi.dedup.....c4d44bbfe60a9b575a60689b2702e495
- Full Text :
- https://doi.org/10.1016/j.vetpar.2009.12.025⟩