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Chemiluminescence of Neutrophils in Patients with Myeloproliferative or Myelodysplastic Hematologic Diseases. Relation to Neutrophil Alkaline Phosphatase Activity
- Source :
- Internal Medicine. 32:204-209
- Publication Year :
- 1993
- Publisher :
- Japanese Society of Internal Medicine, 1993.
-
Abstract
- In hematological diseases such as myeloproliferative disorders (MPD) or myelodysplastic syndromes (MDS), some abnormalities in the chemiluminescence of neutrophils are observed. There are two groups; one includes chronic myelogenous leukemia (CML), essential thrombocythemia (ET) and MDS, which all have decreased chemiluminescence of neutrophils. The other group includes polycythemia vera (PV) which has increased neutrophil chemiluminescence. We studied the neutrophil function by analyzing the chemiluminescence in 35 patients with hematological diseases. In most of these cases the defects in chemiluminescence in 35 patients with hematological diseases. In most of these cases the defects in chemiluminescence in response to N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) were correlated with those in response to phorbol 12-myristate 13-acetate (PMA). But there were exceptional cases in which the maximal light emission of chemiluminescence (Max CL) in response to FMLP was obviously lower than controls despite the fact that the Max CL in response to PMA was the same as the controls. These facts suggest a heterogenicity of the defect site in these diseases. There was a correlation between the level of chemiluminescence and the neutrophil alkaline phosphatase (NAP) activity in these patients. In vitro culture of CML neutrophils with granulocyte colony-stimulating factor (G-CSF) showed a correlation between the increase in the level of chemiluminescence and NAP activity. These results suggest that NAP may take part in the control of neutrophil function.
- Subjects :
- medicine.medical_specialty
Neutrophils
Granulocyte
law.invention
Leukocyte Count
Polycythemia vera
law
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
hemic and lymphatic diseases
Internal medicine
Granulocyte Colony-Stimulating Factor
Internal Medicine
medicine
Humans
Cells, Cultured
Chemiluminescence
Myeloproliferative Disorders
Essential thrombocythemia
business.industry
Myelodysplastic syndromes
General Medicine
Alkaline Phosphatase
medicine.disease
N-Formylmethionine Leucyl-Phenylalanine
Nap
medicine.anatomical_structure
Endocrinology
Myelodysplastic Syndromes
Luminescent Measurements
Immunology
Tetradecanoylphorbol Acetate
Light emission
business
Thrombocythemia, Essential
Chronic myelogenous leukemia
Subjects
Details
- ISSN :
- 13497235 and 09182918
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Internal Medicine
- Accession number :
- edsair.doi.dedup.....c4cd0f76f7c0019969bdec5b442babdb
- Full Text :
- https://doi.org/10.2169/internalmedicine.32.204