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Effect of Fibroblast-Like Cells of Mesenchymal Origin of Cytotoxic Activity of Lymphocytes against NK-Sensitive Target Cells
- Source :
- Bulletin of experimental biology and medicine. 162(4)
- Publication Year :
- 2016
-
Abstract
- We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity. This suggests that fibroblast-like cells can suppress not only isolated NK cells, but also NK cells in natural cell microenvironment.
- Subjects :
- 0301 basic medicine
Stromal cell
Lymphocyte
Primary Cell Culture
Cell Communication
Lymphocyte Activation
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Interleukin 21
Jurkat Cells
medicine
Cytotoxic T cell
Humans
Skin
Lymphokine-activated killer cell
Chemistry
Mesenchymal stem cell
Degranulation
Mesenchymal Stem Cells
General Medicine
Fibroblasts
Fetal Blood
Coculture Techniques
Cell biology
Killer Cells, Natural
030104 developmental biology
medicine.anatomical_structure
Interleukin 12
Interleukin-2
Subjects
Details
- ISSN :
- 15738221
- Volume :
- 162
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Bulletin of experimental biology and medicine
- Accession number :
- edsair.doi.dedup.....c49f5022df3775a9a324ad7a64b3f28f