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Effects of beta 2 adrenergic agonists on axonal injury and mitochondrial metabolism in experimental autoimmune encephalomyelitis rats
- Source :
- Genetics and molecular research : GMR. 14(4)
- Publication Year :
- 2015
-
Abstract
- The primary aims of this study were to investigate mitochondrial metabolism during experimental allergic encephalomyelitis (EAE) animal model axonal injury and to determine the correlation among neurological function scores, pathological changes, and the activities of the BB isoenzyme of creatine kinase (CK-BB), catalase (CAT), and calpain in the brain tissues of EAE rats. Another goal was to preliminarily define the mechanism of mitochondrial metabolism resulting from the effect of beta 2 adrenergic agonists in the process of EAE animal model axonal damage. EAE was induced in specific pathogen free Wistar rats by guinea pig spinal cord homogenate, complete Freund's adjuvant, and pertussis vaccine. We recorded the behavioral change in EAE rats, detected pathological changes in central nervous tissue, and observed the changes of the CK-BB, CAT, and calpain in the EAE rat brain and spinal cord. The results indicated that the average neurologic function score increased in the EAE group compared to that of the controls (P < 0.01). In addition, CAT and CK-BB activities significantly decreased and the calpain activity significantly increased compared with those of the control group (P < 0.05). The decrease of the activity of central nervous CK-BB and CAT content, as well as the increase of calpain activity at the highest time point were considered to be the consequences of EAE. Furthermore, the results revealed that use of salbutamol could alleviate disease symptoms and reduce the recurrence of the EAE disease.
- Subjects :
- medicine.medical_specialty
Encephalomyelitis, Autoimmune, Experimental
Encephalomyelitis
Guinea Pigs
Biology
Guinea pig
Internal medicine
Genetics
medicine
Animals
Rats, Wistar
Molecular Biology
Adrenergic beta-2 Receptor Agonists
Specific-pathogen-free
Calpain
Experimental autoimmune encephalomyelitis
General Medicine
medicine.disease
Spinal cord
Catalase
Axons
Rats
Endocrinology
medicine.anatomical_structure
biology.protein
Creatine kinase
Female
Subjects
Details
- ISSN :
- 16765680
- Volume :
- 14
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Genetics and molecular research : GMR
- Accession number :
- edsair.doi.dedup.....c49d7c072246ecd9791a5a2db4572bad