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Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase
- Source :
- Scopus-Elsevier
- Publication Year :
- 1991
- Publisher :
- American Society for Microbiology, 1991.
-
Abstract
- Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related transposons involves excision of a free, nonreplicative, covalently closed circular intermediate that is substrate for integration. Excisive recombination requires two transposon-encoded proteins, Xis-Tn and Int-Tn, whereas the latter protein alone is sufficient for integration. Here we report that conjugative transposition of Tn916 requires the presence of a functional integrase in both donor and recipient strains. We have constructed a mutant, designated Tn916-int1, by replacing the gene directing synthesis of Int-Tn by an allele inactivated in vitro. In mating experiments, transfer of Tn916-int1 from Bacillus subtilis to E. faecalis was detected only when the transposon-encoded integrase was supplied by trans-complementation in both the donor and the recipient. These results suggest that conjugative transposition of Tn916 requires circularization of the element in the donor followed by transfer and integration of the nonreplicative intermediate in the recipient.
- Subjects :
- DNA, Bacterial
Transposable element
Molecular Sequence Data
Restriction Mapping
Mutant
Bacillus subtilis
Polymerase Chain Reaction
Microbiology
Transposition (music)
Plasmid
stomatognathic system
Enterococcus faecalis
Escherichia coli
Molecular Biology
Recombination, Genetic
Genetics
Base Sequence
Integrases
biology
Genetic transfer
Chromosomes, Bacterial
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Integrase
Conjugation, Genetic
DNA Nucleotidyltransferases
DNA Transposable Elements
biology.protein
bacteria
Oligonucleotide Probes
Plasmids
Research Article
Subjects
Details
- ISSN :
- 10985530 and 00219193
- Volume :
- 173
- Database :
- OpenAIRE
- Journal :
- Journal of Bacteriology
- Accession number :
- edsair.doi.dedup.....c4932a289d546841b6de3fcd0647af41