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Preferential combination between the light and heavy chain isotypes of fish immunoglobulins
- Source :
- Developmental and comparative immunology. 61
- Publication Year :
- 2016
-
Abstract
- Immunoglobulin light chain (IgL) is necessary for the assembly of an Ig molecule, which plays important roles in the immune response. IgL genes were identified in various teleost species, but the basic functions of different IgL isotypes and the preferential combination between IgL and IgH (Ig heavy chain) isotypes remain unclear. In the current study, by EST database searching and cDNA cloning in rainbow trout, 8 IgL sequences were obtained, which could be classified into the IgLκF, IgLκG, IgLσ and IgLλ isotypes, respectively. Trout IgL isotypes were highly expressed in the immune-related tissues, and participated in the immune responses in spleen and gut by stimulation with LPS and poly (I:C). The results of FACS and LC-MS/MS indicated that the IgLκG and IgLσ isotypes preferentially bonded with the heavy chains of IgM and IgT, respectively, in trout B cells and serum. In addition, the genomic organization of trout IgL isotypes and the utilization of recombination signal sequences were studied.
- Subjects :
- 0301 basic medicine
Fish Proteins
Lipopolysaccharides
Trout
animal diseases
Immunology
Immunoglobulin light chain
03 medical and health sciences
0302 clinical medicine
D-segment
Recombination signal sequences
Animals
Cells, Cultured
Genomic organization
Genetics
B-Lymphocytes
biology
Fishes
biology.organism_classification
Molecular biology
Immunoglobulin Isotypes
Intestines
030104 developmental biology
Poly I-C
biology.protein
Immunoglobulin heavy chain
Immunoglobulin Light Chains
Antibody
Immunoglobulin Heavy Chains
J-segment
Dimerization
Spleen
030215 immunology
Developmental Biology
Protein Binding
Subjects
Details
- ISSN :
- 18790089
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....c480ae6d0c9a13c2c6e3553cc7698357