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Inhibition of HIV-1 replication by eIF3f
- Source :
- Proceedings of the National Academy of Sciences. 106:4071-4078
- Publication Year :
- 2009
- Publisher :
- Proceedings of the National Academy of Sciences, 2009.
-
Abstract
- Viruses often use host machinery in unusual ways to execute different steps during their replication. To identify host factors critical for virus replication, we screened cDNA expression libraries for genes or gene fragments that could interfere with HIV-1 vector transduction. The DNA clone that most potently inhibited HIV-1 expression encoded the N-terminal 91 aa of the eukaryotic initiation factor 3 subunit f (N91-eIF3f). Overexpression of N91-eIF3f or full-length eIF3f drastically restricted HIV-1 replication by reducing nuclear and cytoplasmic viral mRNA levels. N91-eIF3f and eIF3f specifically targeted the 3′ long terminal repeat (3′LTR) region in the viral mRNA. We show that the 3′ end cleavage of HIV-1 mRNA precursors is specifically reduced in N91-eIF3f expressing cells. Our results suggest a role of eIF3f in mRNA maturation and that it can specifically interfere with the 3′ end processing of HIV-1 mRNAs.
- Subjects :
- RFC5
Multidisciplinary
Eukaryotic Initiation Factor-3
Terminal Repeat Sequences
EIF4A1
Biological Sciences
Biology
Virus Replication
Molecular biology
Peptide Fragments
DNA replication factor CDT1
Licensing factor
Replication factor C
Viral replication
Transduction, Genetic
HIV-1
biology.protein
Humans
RNA, Viral
Origin recognition complex
Translation factor
Gene Library
Protein Binding
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....c470b281fd4e09e5fec1635e968b765a