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Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin
- Source :
- FEBS letters, 272(1-2), 109-112. Wiley-Blackwell
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) from man, mouse and chicken and recognises a quite different part of the proposed triple-helical region of dystrophin (amino acids 1750–2248). The results suggest that structural homologies between actinin and dystrophin occur more than once in their central helical regions and provide experimental support for an actinin-like central rod model for dystrophin.
- Subjects :
- Gene isoform
musculoskeletal diseases
Monoclonal antibody
pATH vector
Blotting, Western
Biophysics
Actinin
macromolecular substances
Biochemistry
Dystrophin
Epitopes
Mice
Structural Biology
Antibody Specificity
Utrophin
Genetics
Animals
Humans
Molecular Biology
Actin
Antiserum
biology
Muscles
Antibodies, Monoclonal
Cell Biology
Muscular Dystrophy, Animal
musculoskeletal system
Muscular dystrophy
Molecular biology
Molecular Weight
Actinin, alpha 1
Microscopy, Fluorescence
Polyclonal antibodies
biology.protein
Recombinant fusion protein
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....c45af027feb6551995aec2ee914f1660
- Full Text :
- https://doi.org/10.1016/0014-5793(90)80460-Z