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Evaluation Criteria for Chromosome Instability Detection by FISH to Predict Malignant Progression in Premalignant Glottic Laryngeal Lesions
- Source :
- Cancers, 14(13):3260. Multidisciplinary Digital Publishing Institute (MDPI), Cancers, 14, Cancers; Volume 14; Issue 13; Pages: 3260, Cancers, 14, 13
- Publication Year :
- 2022
-
Abstract
- BACKGROUND: The definition of objective, clinically applicable evaluation criteria for FISH 1c/7c in laryngeal precursor lesions for the detection of chromosome instability (CI). Copy Number Variations (CNV) for chromosomes 1 and 7 reflect the general ploidy status of premalignant head and neck lesions and can therefore be used as a marker for CI.METHODS: We performed dual-target FISH for chromosomes 1 and 7 centromeres on 4 µm formalin-fixed, paraffin-embedded tissue sections of 87 laryngeal premalignancies to detect CNVs. Thirty-five normal head and neck squamous cell samples were used as a control. First, the chromosome 7:1 ratio (CR) was evaluated per lesion. The normal range of CRs (≥0.84 ≤ 1.16) was based on the mean CR +/- 3 x SD found in the normal population. Second, the percentage of aberrant nuclei, harboring > 2 chromosomes of chromosome 1 and/or 7 (PAN), was established (cut-off value for abnormal PAN ≥ 10%).RESULTS: PAN showed a stronger correlation with malignant progression than CR (resp. OR 5.6, p = 0.001 and OR 3.8, p = 0.009). PAN combined with histopathology resulted in a prognostic model with an area under the ROC curve (AUC) of 0.75 (s.e. 0.061, sensitivity 71%, specificity 70%).CONCLUSIONS: evaluation criteria for FISH 1c/7c based on PAN ≥ 10% provide the best prognostic information on the risk of malignant progression of premalignant laryngeal lesions as compared with criteria based on the CR. FISH 1c/7c detection can be applied in combination with histopathological assessment.
- Subjects :
- EXPRESSION
RISK
larynx
Cancer Research
CELL CARCINOMAS
chromosome instability
FISH
head and neck cancer
premalignant
dysplasia
IN-SITU HYBRIDIZATION
CANCER
TUMORS
Women's cancers Radboud Institute for Health Sciences [Radboudumc 17]
All institutes and research themes of the Radboud University Medical Center
Oncology
SDG 3 - Good Health and Well-being
RESECTION MARGINS
EPIDEMIOLOGY
HEAD
LUNG
Rare cancers Radboud Institute for Health Sciences [Radboudumc 9]
Subjects
Details
- Language :
- English
- ISSN :
- 20726694
- Database :
- OpenAIRE
- Journal :
- Cancers, 14(13):3260. Multidisciplinary Digital Publishing Institute (MDPI), Cancers, 14, Cancers; Volume 14; Issue 13; Pages: 3260, Cancers, 14, 13
- Accession number :
- edsair.doi.dedup.....c420d33fa270c4d6ed0f527f830449ca