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Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus
- Source :
- BMC Cell Biology, BMC Cell Biology, Vol 19, Iss 1, Pp 1-13 (2018)
- Publication Year :
- 2018
- Publisher :
- BioMed Central, 2018.
-
Abstract
- Background Interactions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an induction of bacterial autophagy in exfoliative sulcular keratinocytes of the gingival sulcus and cultured keratinocytes treated with Porphyromonas gingivalis-originated LPS (PgLPS). Results Exfoliative sulcular keratinocytes showed an induction of autophagy, in addition to increased expression of LPS-mediated factors including lipopolysaccharide-binding protein and toll-like receptors (TLRs), leading to co-localization of bacteria with autophagosomes. In contrast, exfoliative keratinocytes from the free gingiva did not show similar autophagy. Autophagy activity in human cultured keratinocyte cells (HaCaT) was induced by PgLPS, which was dependent partially on the AMP-activated protein kinase (AMPK) pathway via increased intracellular reactive oxygen species (ROS) and was in association with an activation of TLR4 signaling. After incubation of cultured keratinocytes with E.coli BioParticles following PgLPS stimulation, co-localization of bioparticles with autophagosomes was enhanced. Conversely, blockage of autophagy with 3-methyladenin and LPS-binding with polymyxin B led to significant reduction of co-localization of particles with autophagosomes. Conclusion These findings indicate that PgLPS-induced autophagy is at least partially responsible for interaction between bacteria and sulcular keratinocytes in the gingival sulcus.
- Subjects :
- 0301 basic medicine
Keratinocytes
Lipopolysaccharides
Male
Lipopolysaccharide
Gingiva
Biology
Toll-like receptors (TLRs)
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
AMP-activated protein kinase (AMPK)
medicine
Autophagy
Escherichia coli
Humans
Gingival sulcus
lcsh:QH573-671
Receptor
Protein kinase A
Porphyromonas gingivalis
Cells, Cultured
lcsh:Cytology
Adenylate Kinase
Autophagosomes
Reactive oxygen species (ROS)
Epithelial Cells
Cell Biology
biology.organism_classification
Cell biology
Up-Regulation
Toll-Like Receptor 4
HaCaT
030104 developmental biology
medicine.anatomical_structure
chemistry
030220 oncology & carcinogenesis
Female
Keratinocyte
Reactive Oxygen Species
Microtubule-Associated Proteins
Research Article
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14712121
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- BMC Cell Biology
- Accession number :
- edsair.doi.dedup.....c40fe238a666a3487bdd4ab49a934f94