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Implications for invariant natural killer T cell ligands due to the restricted presence of isoglobotrihexosylceramide in mammals
- Publication Year :
- 2016
-
Abstract
- Development of invariant natural killer T (iNKT) cells requires the presentation of lipid ligand(s) by CD1d molecules in the thymus. The glycosphingolipid (GSL) isoglobotrihexosylceramide (iGb3) has been proposed as the natural iNKT cell-selecting ligand in the thymus and to be involved in peripheral activation of iNKT cells by dendritic cells (DCs). However, there is no direct biochemical evidence for the presence of iGb3 in mouse or human thymus or DCs. Using a highly sensitive HPLC assay, the only tissue where iGb3 could be detected in mouse was the dorsal root ganglion (DRG). iGb3 was not detected in other mouse or any human tissues analyzed, including thymus and DCs. Even in mutant mice that store isoglobo-series GSLs in the DRG, we were still unable to detect these GSLs in the thymus. iGb3 is therefore unlikely to be a physiologically relevant iNKT cell-selecting ligand in mouse and humans. A detailed study is now warranted to better understand the nature of iNKT cell-selecting ligand(s) in vivo .
- Subjects :
- Mutant
Biology
Antigens, CD1
Mice
chemistry.chemical_compound
Dorsal root ganglion
In vivo
medicine
Animals
Humans
Invariant natural killer T-cell
Mice, Knockout
Multidisciplinary
Globosides
Globoside
Ligand
Glycosphingolipid
Biological Sciences
Galactosyltransferases
Cell biology
Killer Cells, Natural
Mice, Inbred C57BL
medicine.anatomical_structure
chemistry
CD1D
Immunology
biology.protein
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c3f2fa8207b74c561e260cd31d3dd897