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Promotion of beta-cell differentiation by the alkaloid conophylline in porcine pancreatic endocrine cells
- Source :
- Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 64(3)
- Publication Year :
- 2009
-
Abstract
- We previously found that conophylline, an alkaloid isolated from the leaves of Ervatamia microphylla , induced β-cell differentiation in rat pancreatic acinar carcinoma cells and in cultured fetal rat pancreatic tissue and that it also decreased the blood glucose level in streptozotocin-treated fetal rats. In the present research, we looked into the effect of conophylline on the differentiation of newborn pig pancreatic endocrine cells into insulin-secreting cells. Conophylline potentiated the differentiation of monolayer cells into insulin-producing cells in the presence of nicotinamide in 3 weeks. Next we prepared islet-like cell clusters (ICC). Cononophylline together with nicotinamide also increased the number of insulin-producing cells and the insulin content in ICC in 3–6 weeks. The ICC thus prepared were sensitive to the glucose concentration for the insulin secretion. Conophylline increased the mRNA expression of PDX-1, neurogenin3, neuroD/Beta2, and insulin in ICC. Thus, the vinca alkaloid conophylline potentiated β-cell differentiation in porcine pancreatic endocrine-rich cells in cluster cultures. Pig pancreatic cells are practical candidate for use in transplantation therapy. Conophylline may thus be useful for the large-scale preparation of porcine insulin-producing cells for the regeneration therapy of type-1 diabetes mellitus.
- Subjects :
- Niacinamide
medicine.medical_specialty
Swine
medicine.medical_treatment
Cellular differentiation
Sus scrofa
Drug Evaluation, Preclinical
Enteroendocrine cell
Biology
Islets of Langerhans
Internal medicine
Insulin Secretion
medicine
Animals
Insulin
Vinca Alkaloids
Pancreatic hormone
Cells, Cultured
Pharmacology
NeuroD
Molecular Structure
Reverse Transcriptase Polymerase Chain Reaction
Cell Differentiation
Drug Synergism
General Medicine
Transplantation
B vitamins
Endocrinology
Glucose
Animals, Newborn
Gene Expression Regulation
Cell culture
Biomarkers
Subjects
Details
- ISSN :
- 19506007
- Volume :
- 64
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biomedicinepharmacotherapy = Biomedecinepharmacotherapie
- Accession number :
- edsair.doi.dedup.....c3ebb3a3f6a6b7e90d812945035c1d88