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Alteration of Tight Junction Protein Expression in Dahl Salt-Sensitive Rat Kidney
- Source :
- Kidney & Blood Pressure Research, Vol 42, Iss 6, Pp 951-960 (2017)
- Publication Year :
- 2017
- Publisher :
- Karger Publishers, 2017.
-
Abstract
- Background/Aims: Altered pressure natriuresis is an important mechanism of hypertension, but it remains elusive at the molecular level. We hypothesized that in the kidney, tight junctions (TJs) may have a role in pressure natriuresis because paracellular NaCl transport affects interstitial hydrostatic pressure. Methods: To assess the association of salt-sensitive hypertension with altered renal TJ protein expression, Dahl salt-sensitive (SS) and salt-resistant (SR) rats were put on an 8% NaCl-containing rodent diet for 4 weeks. Systolic blood pressure (SBP) and urine NaCl excretion were measured weekly, and kidneys were harvested for immunoblotting and quantitative PCR analysis at the end of the animal experiments. Results: SBP was significantly higher in SS rats than in SR rats during the first to fourth weeks of the animal experiments. During the first and second week, urinary NaCl excretion was significantly lower in SS rats as compared with SR rats. However, the difference between the two groups vanished at the third and fourth weeks. In the kidney, claudin-4 protein and mRNA were significantly increased in SS rats as compared with SR rats. On the other hand, occludin protein and mRNA were significantly decreased in SS rats as compared with SR rats. The expression of claudin-2, claudin-7, and claudin-8 did not vary significantly between the two groups. Conclusions: In SS rats, SS hypertension was associated with differential changes in renal TJ protein expression. Both upregulation of claudin-4 and downregulation of occludin might increase paracellular NaCl transport in the kidney, resulting in impaired pressure natriuresis in SS rats.
- Subjects :
- medicine.medical_specialty
lcsh:Diseases of the circulatory (Cardiovascular) system
Time Factors
Urinary system
Hydrostatic pressure
030232 urology & nephrology
Blood Pressure
030204 cardiovascular system & hematology
Sodium Chloride
Pressure natriuresis
Occludin
Kidney
lcsh:RC870-923
Natriuresis
Excretion
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
lcsh:Dermatology
Animals
Sodium Chloride, Dietary
Claudin-4
Dahl salt-sensitive rats
Rats, Inbred Dahl
Tight Junction Proteins
Chemistry
urogenital system
General Medicine
lcsh:RL1-803
lcsh:Diseases of the genitourinary system. Urology
Rats
Blood pressure
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
Nephrology
lcsh:RC666-701
Paracellular transport
Claudins
Hypertension
Cardiology and Cardiovascular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 14230143 and 14204096
- Volume :
- 42
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Kidney & Blood Pressure Research
- Accession number :
- edsair.doi.dedup.....c3d903b5535f37539d5533a4b1648360