Back to Search
Start Over
Molecular identification and phylogenetic relationships of trichomonad isolates of galliform birds inferred from nuclear small subunit rRNA gene sequences
- Source :
- Parasitology research. 106(1)
- Publication Year :
- 2009
-
Abstract
- Histomonas meleagridis is the etiological agent of histomonosis or blackhead disease. Recently, genotyping, based on polymerase chain reaction and sequencing of internal transcribed spacer-1 sequences was applied to various isolates originating from fowl. Three genotypes were described: types I and II isolates were associated with clinical disease and probably derived from H. meleagridis, whereas, type III isolates were not disease-associated and likely corresponded to Parahistomonas wenrichi according to morphological observations. However, this latter species has never been characterized at the molecular level and its phylogenetic relationships with other parabasalids remained hypothetical. To confirm the identification of these isolates, small subunit rRNA gene sequences were obtained from representatives of types I, II, and III and analyzed in a broad phylogeny including 64 other parabasalid sequences. From our phylogenetic trees, we confirmed that types I and II isolates were closely related, if not identical, to H. meleagridis, while type III isolates represented P. wenrichi. Both species clustered together with high support. This grouping suggested that speciation leading to these two species inhabiting the same hosts and ecological niche occurred recently in birds. In addition, speciation was likely followed by loss of pathogenicity in P. wenrichi.
- Subjects :
- Molecular Sequence Data
Biology
Parabasalidea
DNA, Ribosomal
Histomonas meleagridis
law.invention
Birds
Phylogenetics
law
medicine
RNA, Ribosomal, 18S
Animals
Cluster Analysis
Genotyping
Protozoan Infections, Animal
Polymerase chain reaction
Phylogeny
Genetics
General Veterinary
Phylogenetic tree
Bird Diseases
Blackhead disease
Genes, rRNA
General Medicine
Sequence Analysis, DNA
Ribosomal RNA
DNA, Protozoan
medicine.disease
biology.organism_classification
Infectious Diseases
Insect Science
Excavata
Parasitology
RNA, Protozoan
Subjects
Details
- ISSN :
- 14321955
- Volume :
- 106
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Parasitology research
- Accession number :
- edsair.doi.dedup.....c3cf45f9d7af2cf885037095d12fce82