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Kinetics of glucose transport and sequestration in lactating bovine mammary glands measured in vivo with a paired indicator/nutrient dilution technique
- Source :
- Journal of Applied Physiology. 99:799-806
- Publication Year :
- 2005
- Publisher :
- American Physiological Society, 2005.
-
Abstract
- To quantify kinetics of mammary glucose utilization in vivo, 24 paired glucose and extracellular indicator ( p-aminohippuric acid) dilution curves across intact bovine mammary glands were obtained after bolus injections into the external iliac artery. Dilution curves were analyzed using a compartmental capillary, convolution integration model. Four candidate submodels of glucose transport and metabolism in capillary supply zones were fit to the glucose dilution curves and evaluated. Model I, with one extracellular compartment for glucose and first-order unidirectional uptake, failed, indicating that efflux of glucose from the intracellular space could not be ignored. Model II, with first-order exchanges between extracellular and intracellular compartments and sequestration from the latter, was overdefined because unidirectional clearance of glucose was at least five times the blood flow rate and 20 times the net clearance rate. Model III, combining extracellular and intracellular space into one compartment, was superior in its goodness-of-fit to curves and identifiability of parameters. Michaelis-Menten parameters of sequestration were not identifiable. Parameters of the optimal compartmental capillary, convolution integration model were applicable to both the dynamics of injected glucose dilution and the steady-state background arteriovenous difference of glucose. Glucose sequestration followed first-order kinetics between 0 and 7 mM extracellular glucose with an average rate constant of 0.006 s−1 or a clearance of 44 ml/s. The ratio of intracellular to extracellular glucose distribution space was 0.34, which is considerably lower than the expected intracellular volume and suggests an intracellular occlusion compartment with which extracellular glucose rapidly exchanges.
- Subjects :
- Metabolic Clearance Rate
Physiology
Glucose uptake
Mammary gland
Kinetics
Biological Transport, Active
Indicator Dilution Techniques
Lactose
Models, Biological
Capillary Permeability
chemistry.chemical_compound
In vivo
Physiology (medical)
Extracellular
medicine
Animals
Lactation
Computer Simulation
Breast
Chemistry
Glucose transporter
Capillaries
Dilution
Glucose
medicine.anatomical_structure
Biochemistry
Cattle
Female
Subjects
Details
- ISSN :
- 15221601 and 87507587
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Physiology
- Accession number :
- edsair.doi.dedup.....c3c7aa57be195f159556a2eb772f3650