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Role of trehalose biosynthesis in environmental survival and virulence of Salmonella enterica serovar typhimurium
- Source :
- Research in Microbiology. 153:281-287
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- The otsA and otsB genes, encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase respectively, have been isolated from Salmonella enterica serovar typhimurium and nucleotide-sequenced. Induction of trehalose biosynthesis by exposure of bacteria to high osmotic strength resulted in the intracellular accumulation of trehalose. An otsA mutant of S. enterica serovar typhimurium was more susceptible to killing by heat, and grew poorly under conditions of high osmolarity. The wild-type and otsA mutant strains showed similar abilities to colonise spleen tissues after oral dosing of mice. These findings suggest that the otsBA gene products play a role in environmental survival, but not in virulence, of S. enterica serovar typhimurium.
- Subjects :
- DNA, Bacterial
Salmonella typhimurium
Hot Temperature
Operon
Mutant
Virulence
Polymerase Chain Reaction
Microbiology
Mice
chemistry.chemical_compound
Osmotic Pressure
Animals
Cloning, Molecular
Molecular Biology
Gene
Mice, Inbred BALB C
Osmotic concentration
biology
Trehalose
General Medicine
biology.organism_classification
Phosphoric Monoester Hydrolases
Mutagenesis, Insertional
chemistry
Glucosyltransferases
Salmonella enterica
Female
Bacteria
Subjects
Details
- ISSN :
- 09232508
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- Research in Microbiology
- Accession number :
- edsair.doi.dedup.....c3c5b92bf1e69cad1f596789a2ebefae