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Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2-biased cytokine profiles in antigen presenting cells
- Source :
- Biochimie, Biochimie, Elsevier, 2019, 167, pp.135-144. ⟨10.1016/j.biochi.2019.09.007⟩, Biochimie (167), 135-144. (2019), Biochimie, 2019, 167, pp.135-144. ⟨10.1016/j.biochi.2019.09.007⟩
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- This work was supported by the the University of Tours (to IDP and FDG), the University of Montpellier (to SD and EC), the Deutsche Forschungsgemeinschaft (to RTS), the Wellcome Trust project grant 093228 (to TKS) and the Campus France/DAAD PHC PROCOPE 24931RE (to RTS and EC). The funding source has no involvement in the conduct of the research and preparation of the article. Glycosylphosphatidylinositols (GPIs) are glycolipids described as toxins of protozoan parasites due to their inflammatory properties in mammalian hosts characterized by the production of interleukin (IL)-1, IL-12 and tumor necrosis factor (TNF)-α. In the present work, we studied the cytokines produced by antigen presenting cells in response to ten different GPI species extracted from Babesia divergens, responsible for babesiosis. Interestingly, B. divergens GPIs induced the production of anti-inflammatory cytokines (IL-2, IL-5) and of the regulatory cytokine IL-10 by macrophages and dendritic cells. In contrast to all protozoan GPIs studied until now, GPIs from B. divergens did not stimulate the production of TNF-α and IL-12, leading to a unique Th1/Th2 profile. Analysis of the carbohydrate composition of the B. divergens GPIs indicated that the di-mannose structure was different from the evolutionary conserved tri-mannose structure, which might explain the particular cytokine profile they induce. Expression of major histocompatibility complex (MHC) molecules on dendritic cells and apoptosis of mouse peritoneal cells were also analysed. B. divergens GPIs did not change expression of MHC class I, but decreased expression of MHC class II at the cell surface, while GPIs slightly increased the percentages of apoptotic cells. During pathogenesis of babesiosis, the inflammation-coagulation auto-amplification loop can lead to thrombosis and the effect of GPIs on coagulation parameters was investigated. Incubation of B. divergens GPIs with rat plasma ex vivo led to increase of fibrinogen levels and to prolonged activated partial thromboplastin time, suggesting a direct modulation of the extrinsic coagulation pathway by GPIs. Publisher PDF
- Subjects :
- [SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology
0301 basic medicine
TAT, Thrombin-antithrombin
Glycosylphosphatidylinositols
QH301 Biology
medicine.medical_treatment
Major histocompatibility complex
Apoptosis
modèle murin
Biochemistry
Antigen presentation
Coagulation
Glycosylphosphatidylinositol
Interleukin
Mice
glycolipide
Immunologie
QD
cytokine proinflammatoire
Babesia divergens
APTT, Activated partial thromboplastin time
AT, Antithrombin
biology
Chemistry
PECs, Peritoneal exudate cells
Microbiology and Parasitology
[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology
Hematology
General Medicine
Microbiologie et Parasitologie
babesia divergens
3. Good health
Cell biology
apicomplexe
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Cytokine
parasite
Cytokines
[SDV.IMM]Life Sciences [q-bio]/Immunology
Tumor necrosis factor alpha
TLR, Toll-like receptor
[SDV.IMM] Life Sciences [q-bio]/Immunology
récepteur de type toll like
Immunology
NDAS
Babesia
Antigens, Protozoan
complexe majeur d'histocompatibilité
Article
RC0254
QH301
03 medical and health sciences
SDG 3 - Good Health and Well-being
Babesiosis
MHC class I
sang
medicine
Animals
Rats, Wistar
Antigen-presenting cell
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Blood Coagulation
MHC class II
030102 biochemistry & molecular biology
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Macrophages
SRDC, Sophie Ruiz dendritic cells
Dendritic Cells
QD Chemistry
biology.organism_classification
Rats
PT, Prothrombin time
030104 developmental biology
biology.protein
Hématologie
Subjects
Details
- ISSN :
- 03009084
- Volume :
- 167
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....c3c551aae7588217034a480107aa5817