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Desorption ionization using through‐hole alumina membrane offers higher reproducibility than 2,5‐dihydroxybenzoic acid, a widely used matrix in Fourier transform ion cyclotron resonance mass spectrometry imaging analysis
- Source :
- Rapid Communications in Mass Spectrometry. 35
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- RATIONALE DIUTHAME (desorption ionization using through-hole alumina membrane), a recently developed matrix-free ionization-assisting substrate, was examined for reproducibility in terms of mass accuracy and intensity using standard lipid and mouse brain sections. The impregnation property of DIUTHAME significantly improved the reproducibility of mass accuracy and intensity compared with 2,5-dihydroxybenzoic acid (DHB). METHODS Frozen tissue sections were mounted on indium tin oxide-coated glass slides. DIUTHAME and DHB were applied to individual sections. Subsequently, a solution of a phosphatidylcholine standard, PC(18:2/18:2), was poured onto the DIUTHAME and matrix. Finally, the samples were subjected to laser desorption ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry. The reproducibility was tested by calculating the mean ± standard deviation values of mass errors and intensities of individual ion species. RESULTS Analysis of the PC(18:2/18:2) standard showed significantly (p
- Subjects :
- Reproducibility
010401 analytical chemistry
Organic Chemistry
Analytical chemistry
Substrate (chemistry)
chemistry.chemical_element
01 natural sciences
Fourier transform ion cyclotron resonance
Mass spectrometry imaging
0104 chemical sciences
Analytical Chemistry
Intensity (physics)
Ion
Matrix (chemical analysis)
chemistry
Spectroscopy
Indium
Subjects
Details
- ISSN :
- 10970231 and 09514198
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Rapid Communications in Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....c3bacca2abde8edb76869ed00cbba628