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Extracellular vesicles of bovine small follicular fluid promote ovarian cortical stromal cell proliferation and steroidogenesis
- Source :
- Reproduction in Domestic Animals. 56:1425-1434
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The aim of this study was to investigate the effects of extracellular vesicles (EVs) on the proliferation and steroid hormone synthesis of bovine ovarian cortical stromal cells in vitro. The release and uptake of EVs are the new mechanisms of cell-to-cell communication. Using reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, TUNEL and other experiments, we found that EVs in bovine follicular fluid can promote the proliferation and synthesis of androstenedione and progesterone in ovarian cortical stromal cells. Moreover, 100 μg/ml EVs caused the most significant effect. We conclude that EVs at 100 μg/ml can significantly promote the proliferation and synthesis of androstenedione and progesterone in ovarian cortical stromal cells. This research is of great significance for further elucidating the regulatory role of follicular fluid EVs in follicular development and atresia and for research on the interaction of ovarian stromal cells, granulosa cells and oocytes.
- Subjects :
- endocrine system
Stromal cell
medicine.medical_treatment
Cell Communication
Extracellular Vesicles
Endocrinology
Follicular phase
medicine
Animals
Androstenedione
Progesterone
Cell Proliferation
Cell growth
Chemistry
Ovary
Follicular fluid
Reverse transcriptase
In vitro
Follicular Fluid
Cell biology
Steroid hormone
Cattle
Female
Animal Science and Zoology
Stromal Cells
Biotechnology
Subjects
Details
- ISSN :
- 14390531 and 09366768
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Reproduction in Domestic Animals
- Accession number :
- edsair.doi.dedup.....c3a5b65b0e5bbda776395c5083efa9a6