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Clinical impact of real-time evaluation of the biological activity and degradation of hepatocyte growth factor
- Source :
- Growth Factors (Chur, Switzerland)
- Publication Year :
- 2008
- Publisher :
- Informa Healthcare, 2008.
-
Abstract
- Hepatocyte growth factor (HGF) is essential for injury repair. Despite high HGF levels in chronic ulcers, up-regulation of HGF receptor in ulcer tissue and decreased biological activity of HGF in ulcer secretions have been observed. With a surface plasmon resonance-based method, we assessed the binding of HGF to antibodies, receptors, and the basement membrane and identified binding interactions that are indispensable for the biological activity of HGF. Recombinant HGF (rHGF) lots were tested for activity, structural integrity, and degradation, and the results were verified in an in vitro model of cell injury. Biologically active rHGF, as well as plasma from healthy volunteers, bound to heparan sulphate proteoglycan (HSPG) and to anti-HGF antibodies. Decreased binding to HSPG was the first event in rHGF degradation. This study established the feasibility of identifying patients with chronic inflammation who need exogenous HGF and of using ligand-binding assessment to evaluate rHGF lots for biological activity.
- Subjects :
- Adult
Male
Clinical Biochemistry
SPR
Inflammation
biological activity
Plasma protein binding
Biology
Basement Membrane
Endocrinology
Cell Line, Tumor
medicine
Humans
HGF
Receptor
Ulcer
Aged
Basement membrane
Aged, 80 and over
Wound Healing
Hepatocyte Growth Factor
Biological activity
Cell Biology
Middle Aged
Surface Plasmon Resonance
Recombinant Proteins
Cell biology
medicine.anatomical_structure
inflammation
Immunology
biology.protein
Hepatocyte growth factor
Original Article
chronic ulcer
Female
medicine.symptom
Antibody
Wound healing
Heparan Sulfate Proteoglycans
medicine.drug
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 10292292 and 08977194
- Volume :
- 26
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Growth Factors (Chur, Switzerland)
- Accession number :
- edsair.doi.dedup.....c39514b22b6db59e54c583b4c437ea82