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Clinical impact of real-time evaluation of the biological activity and degradation of hepatocyte growth factor

Authors :
Daniel Aili
Lars Brudin
Tayeb Nayeri
Bo Liedberg
Fariba Nayeri
Source :
Growth Factors (Chur, Switzerland)
Publication Year :
2008
Publisher :
Informa Healthcare, 2008.

Abstract

Hepatocyte growth factor (HGF) is essential for injury repair. Despite high HGF levels in chronic ulcers, up-regulation of HGF receptor in ulcer tissue and decreased biological activity of HGF in ulcer secretions have been observed. With a surface plasmon resonance-based method, we assessed the binding of HGF to antibodies, receptors, and the basement membrane and identified binding interactions that are indispensable for the biological activity of HGF. Recombinant HGF (rHGF) lots were tested for activity, structural integrity, and degradation, and the results were verified in an in vitro model of cell injury. Biologically active rHGF, as well as plasma from healthy volunteers, bound to heparan sulphate proteoglycan (HSPG) and to anti-HGF antibodies. Decreased binding to HSPG was the first event in rHGF degradation. This study established the feasibility of identifying patients with chronic inflammation who need exogenous HGF and of using ligand-binding assessment to evaluate rHGF lots for biological activity.

Details

Language :
English
ISSN :
10292292 and 08977194
Volume :
26
Issue :
3
Database :
OpenAIRE
Journal :
Growth Factors (Chur, Switzerland)
Accession number :
edsair.doi.dedup.....c39514b22b6db59e54c583b4c437ea82