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Cytoglobin modulates myogenic progenitor cell viability and muscle regeneration
- Source :
- Proceedings of the National Academy of Sciences. 111
- Publication Year :
- 2013
- Publisher :
- Proceedings of the National Academy of Sciences, 2013.
-
Abstract
- Mammalian skeletal muscle can remodel, repair, and regenerate itself by mobilizing satellite cells, a resident population of myogenic progenitor cells. Muscle injury and subsequent activation of myogenic progenitor cells is associated with oxidative stress. Cytoglobin is a hemoprotein expressed in response to oxidative stress in a variety of tissues, including striated muscle. In this study, we demonstrate that cytoglobin is up-regulated in activated myogenic progenitor cells, where it localizes to the nucleus and contributes to cell viability. siRNA-mediated depletion of cytoglobin from C2C12 myoblasts increased levels of reactive oxygen species and apoptotic cell death both at baseline and in response to stress stimuli. Conversely, overexpression of cytoglobin reduced reactive oxygen species levels, caspase activity, and cell death. Mice in which cytoglobin was knocked out specifically in skeletal muscle were generated to examine the role of cytoglobin in vivo. Myogenic progenitor cells isolated from these mice were severely deficient in their ability to form myotubes as compared with myogenic progenitor cells from wild-type littermates. Consistent with this finding, the capacity for muscle regeneration was severely impaired in mice deficient for skeletal-muscle cytoglobin. Collectively, these data demonstrate that cytoglobin serves an important role in muscle repair and regeneration.
- Subjects :
- Male
Time Factors
Satellite Cells, Skeletal Muscle
Cell Survival
Cellular differentiation
Apoptosis
Biology
Cell Line
Mice
medicine
Animals
Regeneration
Myocyte
RNA, Small Interfering
Progenitor cell
Cell Proliferation
Mice, Knockout
Multidisciplinary
Myogenesis
Muscles
Stem Cells
Cytoglobin
Skeletal muscle
Cell Differentiation
Immunohistochemistry
Globins
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
PNAS Plus
Gene Expression Regulation
Stem cell
Reactive Oxygen Species
C2C12
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....c389978181e979e0b6fb2488d3a0cd81