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Accelerated Experience-Dependent Pruning of Cortical Synapses in Ephrin-A2 Knockout Mice
Accelerated Experience-Dependent Pruning of Cortical Synapses in Ephrin-A2 Knockout Mice
- Source :
- Neuron, vol 80, iss 1
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- SummaryRefinement of mammalian neural circuits involves substantial experience-dependent synapse elimination. Using in vivo two-photon imaging, we found that experience-dependent elimination of postsynaptic dendritic spines in the cortex was accelerated in ephrin-A2 knockout (KO) mice, resulting in fewer adolescent spines integrated into adult circuits. Such increased spine removal in ephrin-A2 KOs depended on activation of glutamate receptors, as blockade of the N-methyl-D-aspartate (NMDA) receptors eliminated the difference in spine loss between wild-type and KO mice. We also showed that ephrin-A2 in the cortex colocalized with glial glutamate transporters, which were significantly downregulated in ephrin-A2 KOs. Consistently, glial glutamate transport was reduced in ephrin-A2 KOs, resulting in an accumulation of synaptic glutamate. Finally, inhibition of glial glutamate uptake promoted spine elimination in wild-type mice, resembling the phenotype of ephrin-A2 KOs. Together, our results suggest that ephrin-A2 regulates experience-dependent, NMDA receptor-mediated synaptic pruning through glial glutamate transport during maturation of the mouse cortex.
- Subjects :
- Dendritic spine
Neuroscience(all)
Knockout
1.1 Normal biological development and functioning
Amino Acid Transport System X-AG
Dendritic Spines
Synaptic pruning
Biology
Neurotransmission
Receptors, N-Methyl-D-Aspartate
Synaptic Transmission
Article
Mice
Underpinning research
Postsynaptic potential
Receptors
medicine
Psychology
Animals
Mice, Knockout
Neurology & Neurosurgery
General Neuroscience
Neurosciences
Glutamate receptor
Brain
Ephrin-A2
Excitatory Postsynaptic Potentials
medicine.anatomical_structure
nervous system
Neurological
Synapses
Excitatory postsynaptic potential
NMDA receptor
Neuroglia
Cognitive Sciences
sense organs
Neuroscience
N-Methyl-D-Aspartate
Subjects
Details
- ISSN :
- 08966273
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....c35bc274f40da27df3d3e17bc693f842
- Full Text :
- https://doi.org/10.1016/j.neuron.2013.07.014