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High-performance liquid chromatography with electrochemical detection of gossypol in human plasma
- Source :
- Journal of Chromatography B: Biomedical Sciences and Applications. 343:387-396
- Publication Year :
- 1985
- Publisher :
- Elsevier BV, 1985.
-
Abstract
- A sensitive and selective high-performance liquid chromatographic method with electrochemical detection for the determination of gossypol in human plasma is described. Glutathione is used as a protective agent and gossypol dimethyl ether as an internal standard. Acetonitrile-treated protein-free plasma sample is first introduced on to a C18 pre-column for enrichment and clean-up. By using a column-switching technique, gossypol and the internal standard are subjected to further separation on a C8 analytical column, while the major interfering components are eliminated before entering the column. Methanol-0.1 M citrate buffer (pH 3.2) (80:20) is used as the mobile phase. The detector potential on the glassy carbon electrode is maintained at +0.6 V vs. an Ag-AgCl reference electrode. The linearity with human plasma ranged from 5 to 250 ng/ml. The absolute recoveries of gossypol and gossypol dimethyl ether were 91.3 and 97.5%, respectively, with a within-day precision of 2.5% and a day-to-day precision of 3.8%. The limit of detection is 5 ng/ml (signal-to-noise ratio = 3:1). The method is considered to be suitable for the clinical pharmacokinetic studies of gossypol.
- Subjects :
- Detection limit
Chromatography
Gossypol
Temperature
General Chemistry
Glutathione
Electrochemical detection
High-performance liquid chromatography
Reference electrode
Kinetics
chemistry.chemical_compound
Drug Stability
chemistry
Phase (matter)
Electrochemistry
Humans
Indicators and Reagents
Dimethyl ether
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 03784347
- Volume :
- 343
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B: Biomedical Sciences and Applications
- Accession number :
- edsair.doi.dedup.....c3545dc57ec76eaca5e709b6d2844ec7