Preliminary investigation of crystals of the neutral lipase from Pseudomonas fluorescens

The neutral lipase from the bacteria Pseudomonas fluorescens, marketed under the trade name LpL-200S, has been crystallized in a form suitable for X-ray diffraction analysis from 35% n-propanol at pH 8·5. The crystals are monoclinic prisms and are of space group C2 with a = 91·00 A ̊, b = 47·17 A ̊, c = 35·21 A ̊ and β = 121·43 °. There is one molecule of the protein as the asymmetric unit of the crystals. The diffraction pattern extends to at least 1·6 Å resolution and the crystals are extremely robust in terms of X-ray exposure. © 1991.