Back to Search
Start Over
Characterization of berberine transport into Coptis japonica cells and the involvement of ABC protein
- Source :
- Scopus-Elsevier
- Publication Year :
- 2002
- Publisher :
- Oxford University Press (OUP), 2002.
-
Abstract
- Cultured Coptis japonica cells are able to take up berberine, a benzylisoquinoline alkaloid, from the medium and transport it exclusively into the vacuoles. Uptake activity depends on the growth phase of the cultured cells whereas the culture med ium had no effect on uptake. Treatment with several inhibitors suggested that berberine uptake depended on the ATP level. Some inhibitors of P-glycoprotein, an ABC transporter involved in multiple drug resist ance in cancer cells, strongly inhibited berberine uptake, whereas a specific inhibitor for glutathione biosynthesis and vacuolar ATPase, bafilomycin A1, had little effect. Vanadate-induced ATP trap experi ments to detect ABC proteins expressed in C. japon ica cells showed that three membrane proteins of between 120 and 150 kDa were photolabelled with 8-azido-[a-32P] ATP. Two revealed the same photo affinity-labelling pattern as P-glycoprotein, and the interaction of these proteins with berberine was also demonstrated. These results suggest that ABC proteins of the MDR-type are involved in the uptake of berberine from the medium.
- Subjects :
- Azides
Berberine
Nifedipine
Physiology
ATP-binding cassette transporter
Plant Science
Vacuole
Biology
chemistry.chemical_compound
Tobacco
Benzylisoquinoline
Cells, Cultured
Lithospermum
Alkaloid
Bafilomycin
Biological Transport
Hydrogen-Ion Concentration
Quinidine
Anti-Bacterial Agents
Biochemistry
chemistry
Membrane protein
Vacuoles
Cancer cell
Cyclosporine
ATP-Binding Cassette Transporters
Macrolides
Genes, MDR
Vanadates
Ranunculaceae
Subjects
Details
- ISSN :
- 14602431
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....c3342253fc92250f2f640099980665fb