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Theoretical and experimental investigation of the focusing position in asymmetrical flow field-flow fractionation (AF4)
- Source :
- Journal of Chromatography A, Journal of Chromatography A, Elsevier, 2018, 1561, pp.67-75. ⟨10.1016/j.chroma.2018.04.056⟩
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- International audience; To better understand the focusing process and to determine the focusing position (z(foc)) in Asymmetrical Flow Field-Flow Fractionation (AF4), theoretical and experimental studies were performed. A new theoretical expression that allows the calculation of z(foc) in AF4 channels of any shape having an axial plane of symmetry was established. The equation was then applied to the particular case of a trapezoidal AF4 channel with tapered ends. The applicability of this equation was verified by comparing the calculated results with the experimental measures of the focusing position obtained using blue dextran dye under different flow conditions. The calculated values and the experimental results were in good agreement. The total uncertainty associated with the calculated z(foc) was evaluated and was lower than 10%. A good repeatability and reproducibility of the focusing position was obtained.Moreover, the effects of the sample-injection position, the spacer thickness, the membrane aging, and the nature of the injected sample on the focusing position were investigated: z(foc) was not influenced by these parameters, proving the universal applicability of the equation given in this work for predicting the focusing position, when the assumption of constancy of the cross-flow velocity at the membrane, which underlies this equation, is fulfilled. Additionally, it is notable that this theoretical expression is suitable for different models of AF4 channel and not peculiar to specific suppliers
- Subjects :
- Work (thermodynamics)
Focalization
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
Position (vector)
Coloring Agents
Reproducibility
Asymmetrical flow field-flow fractionation
Chromatography
Chemistry
010401 analytical chemistry
Organic Chemistry
Dextrans
General Medicine
Mechanics
Repeatability
Models, Theoretical
021001 nanoscience & nanotechnology
Fractionation, Field Flow
Symmetry (physics)
Expression (mathematics)
0104 chemical sciences
Transverse plane
Flow (mathematics)
[SDU]Sciences of the Universe [physics]
Focusing position
Trapezoidal channel
0210 nano-technology
Subjects
Details
- ISSN :
- 00219673 and 18733778
- Volume :
- 1561
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....c3334ecd7b661ebde68d0ff82800bbe1