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Escherichia coli ST8196 is a novel, locally evolved, and extensively drug resistant pathogenic lineage within the ST131 clonal complex
- Source :
- Emerging Microbes & Infections, article-version (VoR) Version of Record
- Publication Year :
- 2020
- Publisher :
- Informa UK Limited, 2020.
-
Abstract
- The H30Rx subclade of Escherichia coli ST131 is a clinically important, globally dispersed pathogenic lineage that typically displays resistance to fluoroquinolones and extended spectrum β-lactams. Isolates EC233 and EC234, variants of ST131-H30Rx with a novel sequence type (ST) 8196, isolated from unrelated patients presenting with bacteraemia at a Sydney Hospital in 2014 are characterised here. EC233 and EC234 are phylogroup B2, serotype O25:H4A, and resistant to ampicillin, amoxicillin, cefoxitin, ceftazidime, ceftriaxone, ciprofloxacin, norfloxacin and gentamicin and are likely clonal. Both harbour an IncFII_2 plasmid (pSPRC_Ec234-FII) that carries most of the resistance genes on an IS26 associated translocatable unit, two small plasmids and a novel IncI1 plasmid (pSPRC_Ec234-I). SNP-based phylogenetic analysis of the core genome of representatives within the ST131 clonal complex places both isolates in a subclade with three clinical Australian ST131-H30Rx clade-C isolates. A MrBayes phylogeny analysis of EC233 and EC234 indicates ST8196 share a most recent common ancestor with ST131-H30Rx strain EC70 isolated from the same hospital in 2013. Our study identified genomic hallmarks that define the ST131-H30Rx subclade in the ST8196 isolates and highlights a need for unbiased genomic surveillance approaches to identify novel high-risk MDR E. coli pathogens that impact healthcare facilities.
- Subjects :
- 0301 basic medicine
Serotype
Lineage (genetic)
Epidemiology
030106 microbiology
Immunology
E. coli ST131 clonal complex
Drug resistance
Biology
medicine.disease_cause
Microbiology
Genome
03 medical and health sciences
Plasmid
Virology
Drug Discovery
Escherichia coli ST8196
medicine
Escherichia coli
Genetics
Phylogenetic tree
qnrB4
Subclade
Articles
General Medicine
bla CTX-M-15
ST131- H30Rx
030104 developmental biology
Infectious Diseases
Parasitology
Research Article
Subjects
Details
- ISSN :
- 22221751
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Emerging Microbes & Infections
- Accession number :
- edsair.doi.dedup.....c327ca1896a2b68ab97031c62a2d9146