Back to Search
Start Over
Improved microRNA quantification in total RNA from clinical samples
- Source :
- Methods. 50:S6-S9
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- microRNAs are small regulatory RNAs that are currently emerging as new biomarkers for cancer and other diseases. In order for biomarkers to be useful in clinical settings, they should be accurately and reliably detected in clinical samples such as formalin fixed paraffin embedded (FFPE) sections and blood serum or plasma. These types of samples represent a challenge in terms of microRNA quantification. A newly developed method for microRNA qPCR using Locked Nucleic Acid (LNA)-enhanced primers enables accurate and reproducible quantification of microRNAs in scarce clinical samples. Here we show that LNA-based microRNA qPCR enables biomarker screening using very low amounts of total RNA from FFPE samples and the results are compared to microarray analysis data. We also present evidence that the addition of a small carrier RNA prior to total RNA extraction, improves microRNA quantification in blood plasma and laser capture microdissected (LCM) sections of FFPE samples.
- Subjects :
- Paraffin Embedding
Formalin fixed paraffin embedded
Microarray analysis techniques
Lasers
Total rna
RNA
Biology
Polymerase Chain Reaction
Molecular biology
General Biochemistry, Genetics and Molecular Biology
Fixatives
MicroRNAs
Blood serum
Formaldehyde
microRNA
Humans
Biomarker (medicine)
Locked nucleic acid
Molecular Biology
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 10462023
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Methods
- Accession number :
- edsair.doi.dedup.....c3236064c5fb5cb2b6a0143213369167