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Detection of 14q32 translocations in B-cell malignancies by in situ hybridization with yeast artificial chromosome clones containing the human IgH gene locus
- Source :
- Blood. 83:2962-2969
- Publication Year :
- 1994
- Publisher :
- American Society of Hematology, 1994.
-
Abstract
- Partner sites of 14q32 translocations found in B-cell malignancies were detected by fluorescence in situ hybridization (FISH) using yeast artificial chromosome (YAC) clones, Y20 and Y6, containing the human Ig heavy chain (IgH) gene locus. Y20 spans a 160-kb upstream and 40-kb downstream region of the JH segments on chromosome band 14q32.33. Y6 is 300-kb upstream of Y20, and spans a further 320-kb telomeric region. The human DNA sequences amplified by Alu polymerase chain reaction of the YAC clones were used as probes for FISH to study six patients with non-Hodgkin's lymphoma (NHL), one patient with acute lymphoblastic leukemia, and one cell line FR4 established from a plasmacytoma. Three telomeric YAC clones each specific for 3q, 8q, and 18q were also used to further characterize 14q32 translocations. The IgH YACs were successfully applied to detect cytogenetically invisible subtelomeric translocation of the IgH gene locus to each partner site in t(14;18), t(8;14), and t(14;19), and to identify t(3;14) (q27;q32.33) in three patients with 14q32 translocation of unknown origin. Furthermore, complex translocations involving more than three chromosomes were detected in an NHL patient with t(8;14), and t(3;12), and in the FR4 with der(14)t(8;14), der(8)dic(1;8), and del(1)(q21). The technique would be a useful tool in elucidating the mechanisms of a 14q32 translocation in B-cell malignancies.
- Subjects :
- Male
Yeast artificial chromosome
medicine.medical_specialty
Lymphoma, B-Cell
Immunology
Locus (genetics)
Chromosomal translocation
Biology
Biochemistry
Translocation, Genetic
law.invention
law
hemic and lymphatic diseases
medicine
Humans
Child
Chromosomes, Artificial, Yeast
In Situ Hybridization, Fluorescence
Polymerase chain reaction
B cell
Aged
Chromosomes, Human, Pair 14
Genetics
Genes, Immunoglobulin
medicine.diagnostic_test
Cytogenetics
Chromosome Mapping
Cell Biology
Hematology
Middle Aged
Subtelomere
Molecular biology
medicine.anatomical_structure
Female
Immunoglobulin Heavy Chains
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....c2f47557d32795bce1c9e4bfc7b516be