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A Meta-Analysis of Wolbachia Transcriptomics Reveals a Stage-Specific Wolbachia Transcriptional Response Shared Across Different Hosts
- Source :
- G3: Genes, Genomes, Genetics, Vol 10, Iss 9, Pp 3243-3260 (2020)
- Publication Year :
- 2020
- Publisher :
- Oxford University Press, 2020.
-
Abstract
- Wolbachia is a genus containing obligate, intracellular endosymbionts with arthropod and nematode hosts. Numerous studies have identified differentially expressed transcripts in Wolbachia endosymbionts that potentially inform the biological interplay between these endosymbionts and their hosts, albeit with discordant results. Here, we re-analyze previously published Wolbachia RNA-Seq transcriptomics data sets using a single workflow consisting of the most up-to-date algorithms and techniques, with the aim of identifying trends or patterns in the pan-Wolbachia transcriptional response. We find that data from one of the early studies in filarial nematodes did not allow for robust conclusions about Wolbachia differential expression with these methods, suggesting the original interpretations should be reconsidered. Across datasets analyzed with this unified workflow, there is a general lack of global gene regulation with the exception of a weak transcriptional response resulting in the upregulation of ribosomal proteins in early larval stages. This weak response is observed across diverse Wolbachia strains from both nematode and insect hosts suggesting a potential pan-Wolbachia transcriptional response during host development that diverged more than 700 million years ago.
- Subjects :
- RNA-Seq
Biology
QH426-470
Transcriptome
03 medical and health sciences
transcriptomics
0302 clinical medicine
Ribosomal protein
parasitic diseases
Genetics
bacteria
Molecular Biology
Genetics (clinical)
reproductive and urinary physiology
030304 developmental biology
Regulation of gene expression
0303 health sciences
wolbachia
Obligate
Host (biology)
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
intracellular
meta-analysis
Nematode
Evolutionary biology
rna-seq
Wolbachia
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- G3: Genes, Genomes, Genetics
- Accession number :
- edsair.doi.dedup.....c2ec56a5597d4364438ce2bcc2d17732