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Enhancement of Ethanol Production via Hyper Thermal Acid Hydrolysis and Co-Fermentation Using Waste Seaweed from Gwangalli Beach, Busan, Korea
- Source :
- Journal of Microbiology and Biotechnology. 28:401-408
- Publication Year :
- 2018
- Publisher :
- Journal of Microbiology and Biotechnology, 2018.
-
Abstract
- The waste seaweed from Gwangalli beach, Busan, Korea was utilized as biomass for ethanol production. Sagassum fulvellum (brown seaweed, Mojaban in Korean name) comprised 72% of the biomass. The optimal hyper thermal acid hydrolysis conditions were obtained as 8% slurry contents, 138 mM sulfuric acid, and 160oC of treatment temperature for 10 min with a low content of inhibitory compounds. To obtain more monosaccharides, enzymatic saccharification was carried out with Viscozyme L for 48 h. After pretreatment, 34 g/l of monosaccharides were obtained. Pichia stipitis and Pichia angophorae were selected as optimal co-fermentation yeasts to convert all of the monosaccharides in the hydrolysate to ethanol. Co-fermentation was carried out with various inoculum ratios of P. stipitis and P. angophorae. The maximum ethanol concentration of 16.0 g/l was produced using P. stipitis and P. angophorae in a 3:1 inoculum ratio, with an ethanol yield of 0.47 in 72 h. Ethanol fermentation using yeast co-culture may offer an efficient disposal method for waste seaweed while enhancing the utilization of monosaccharides and production of ethanol.
- Subjects :
- 0106 biological sciences
Co-fermentation
Hot Temperature
Biomass
Saccharomyces cerevisiae
010501 environmental sciences
Ethanol fermentation
01 natural sciences
Applied Microbiology and Biotechnology
Pichia
Hydrolysate
Hydrolysis
Bioreactors
010608 biotechnology
Republic of Korea
Monosaccharide
Ethanol fuel
Food science
Pichia stipitis
0105 earth and related environmental sciences
Waste Products
chemistry.chemical_classification
Ethanol
biology
Monosaccharides
General Medicine
Hydrogen-Ion Concentration
Sulfuric Acids
Seaweed
biology.organism_classification
Coculture Techniques
Enzymes
chemistry
Biofuels
Fermentation
Acids
Biotechnology
Subjects
Details
- ISSN :
- 17388872 and 10177825
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....c2e312638fc8579d5167fda5ad0bd20a